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首页> 外文期刊>The Journal of Antimicrobial Chemotherapy >Evaluation of a DNA microarray for the rapid detection of extended-spectrum β-lactamases (TEM, SHV and CTX-M), plasmid-mediated cephalosporinases (CMY-2-like, DHA, FOX, ACC-1, ACT/MIR and CMY-1-like/MOX) and carbapenemases (KPC, OXA-48, VIM, IMP and NDM)
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Evaluation of a DNA microarray for the rapid detection of extended-spectrum β-lactamases (TEM, SHV and CTX-M), plasmid-mediated cephalosporinases (CMY-2-like, DHA, FOX, ACC-1, ACT/MIR and CMY-1-like/MOX) and carbapenemases (KPC, OXA-48, VIM, IMP and NDM)

机译：评估用于快速检测超广谱β-内酰胺酶（TEM，SHV和CTX-M），质粒介导的头孢菌素酶（CMY-2-like，DHA，FOX，ACC-1，ACT / MIR和CMY）的DNA芯片的评估-1-like / MOX）和碳青霉烯酶（KPC，OXA-48，VIM，IMP和NDM）

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Objectives: Carbapenem-resistant Gram-negative bacilli are reported increasingly and represent an emerging public health concern. Laboratory detection of extended-spectrum β-lactamase (ESBL), plasmid-mediated cephalosporinase (pAmpC) and carbapenemase producers remains a challenge for microbiology laboratories and is important to avoid clinical failure due to inappropriate antimicrobial therapy and to prevent nosocomial outbreaks. We evaluated a novel microarray, the 'Check-MDR CT103 array' test (Check-Points, Wageningen, The Netherlands), that employs highly specific DNA markers to identify the β-lactamase genes of ESBLs (TEM, SHV and CTX-M, and discriminates between ESBL and non-ESBL TEM and SHV variants), of pAmpC (CMY-2-like, DHA, FOX, ACC-1, ACT/MIR and CMY-1-like/MOX) and of carbapenemases (KPC, OXA-48, VIM, IMP and NDM). Methods: One-hundred-and-eighty-seven well-characterized Gram-negative bacilli isolates possessing different bla genes were tested. Total DNAs were extracted using a Qiagen DNA mini kit. The 'Check-MDR CT103 array' was used as recommended by the manufacturer. Results: The system correctly identified representatives of the three ESBL gene families tested, including differentiation between non-ESBL and ESBL TEM and SHV variants. All bla CTX-M genes were classified into the appropriate family group (i.e. CTX-M-1 group, CTX-M-2 group, CTX-M-9 group and CTX-M-8/25/26 group). In addition, the clinically relevant plasmid-encoded cephalosporinase and carbapenemase genes were also reliably detected. Specificities and sensitivities of 100% were recorded for most bla genes. Conclusions: The 'Check-MDR CT103 array' is a powerful high-throughput tool for rapid identification of ESBL, pAmpC and carbapenemase producers in culture. Because of its rapid performance, this platform is a valuable tool for epidemiological or infection control studies.
机译：目标：耐碳青霉烯类革兰氏阴性杆菌的报道越来越多，代表了新出现的公共卫生问题。实验室检测广谱β-内酰胺酶（ESBL），质粒介导的头孢菌素酶（pAmpC）和碳青霉烯酶生产者仍然是微生物实验室的挑战，对于避免由于不适当的抗微生物治疗而导致的临床失败以及防止医院爆发是非常重要的。我们评估了一种新型的微阵列“ Check-MDR CT103阵列”测试（Check-Points，瓦格宁根，荷兰），该测试采用高度特异性的DNA标记物来识别ESBLs（TEM，SHV和CTX-M，并区分ESBL和非ESBL TEM和SHV变体，pAmpC（CMY-2-样，DHA，FOX，ACC-1，ACT / MIR和CMY-1-样/ MOX）和碳青霉烯酶（KPC，OXA -48，VIM，IMP和NDM）。方法：测试了一百八十七种具有不同bla基因的革兰氏阴性杆菌。使用Qiagen DNA mini试剂盒提取总DNA。按照制造商的建议使用了“ Check-MDR CT103阵列”。结果：系统正确识别了测试的三个ESBL基因家族的代表，包括非ESBL和ESBL TEM与SHV变异体之间的区分。将所有bla CTX-M基因分类为适当的家族组（即，CTX-M-1组，CTX-M-2组，CTX-M-9组和CTX-M-8 / 25/26组）。此外，还可以可靠地检测到临床相关的质粒编码的头孢菌素酶和碳青霉烯酶基因。对大多数bla基因记录的特异性和敏感性为100％。结论：“ Check-MDR CT103阵列”是一种功能强大的高通量工具，可用于快速鉴定培养物中的ESBL，pAmpC和碳青霉烯酶生产商。由于其快速的性能，该平台是流行病学或感染控制研究的宝贵工具。

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