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首页> 外文期刊>The Journal of General and Applied Microbiology >Isolation of lactic acid bacteria exhibiting high scavenging activity for environmental hydrogen peroxide from fermented foods and its two scavenging enzymes for hydrogen peroxide
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Isolation of lactic acid bacteria exhibiting high scavenging activity for environmental hydrogen peroxide from fermented foods and its two scavenging enzymes for hydrogen peroxide

机译：从发酵食品中分离出对环境中的过氧化氢具有高清除活性的乳酸菌及其对过氧化氢的两种清除酶

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摘要

To obtain lactic acid bacteria that scavenge environmental hydrogen peroxide, we developed a specialized enrichment medium and successfully isolated Pediococcus pentosaceus Be1 strain from a fermented food. This strain showed vigorous environmental hydrogen peroxide scavenging activity over a wide range of hydrogen peroxide concentrations. High Mn-catalase and NADH peroxidase activities were found in the cell-free extract of the P. pentosaceus Bel strain, and these two hydrogen peroxide scavenging enzymes were purified from the cell-free extract of the strain. Mn-catalase has been purified from several microorganisms by several researchers, and the NADH peroxidase was first purified from the original strain in this report. After cloning the genes of the Mn-catalase and the NADH peroxidase, the deduced amino acid sequences were compared with those of known related enzymes.
机译：为了获得清除环境过氧化氢的乳酸菌，我们开发了一种专门的富集培养基，并成功地从发酵食品中分离了戊糖片球菌Be1菌株。该菌株在广泛的过氧化氢浓度范围内表现出强烈的环境过氧化氢清除活性。在P. pentosaceus Bel菌株的无细胞提取物中发现了高的Mn-过氧化氢酶和NADH过氧化物酶活性，并且从该菌株的无细胞提取物中纯化了这两种过氧化氢清除酶。 Mn-过氧化氢酶已经由数名研究人员从几种微生物中纯化出来，而NADH过氧化物酶是在本报告中首先从原始菌株中纯化得到的。在克隆了Mn-过氧化氢酶和NADH过氧化物酶的基因后，将推导的氨基酸序列与已知的相关酶进行了比较。

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