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The role of mass spectrometry in proteome studies

机译:质谱在蛋白质组学研究中的作用

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Mass spectrometry (MS) is an important tool in modern protein chemistry. In proteome analyses the expression of hundreds or thousands of proteins can be monitored at the same time. First, complex protein mixtures are separated by two-dimensional gel electrophoresis (2-DE) and then individual proteins are identified by using MS followed by database searches. Recent developments in this field have made it possible to do automated, high-throughput protein identification that is needed in proteome analyses. MS can also be used to characterize post-translational modifications in proteins and to study protein complexes. This review will introduce the current MS methods used in proteome studies, and discuss their advantages and disadvantages. New instrumental MS developments are also presented that are useful in these analyses.
机译:质谱(MS)是现代蛋白质化学中的重要工具。在蛋白质组分析中,可以同时监视成百上千种蛋白质的表达。首先,通过二维凝胶电泳(2-DE)分离复杂的蛋白混合物,然后使用MS识别单个蛋白,然后进行数据库搜索。该领域的最新发展使蛋白质组学分析所需的自动化,高通量蛋白质鉴定成为可能。 MS还可以用于表征蛋白质中的翻译后修饰并研究蛋白质复合物。这篇综述将介绍蛋白质组学研究中当前使用的质谱方法,并讨论它们的优缺点。还介绍了新的仪器质谱开发方法,这些方法在这些分析中很有用。

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