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首页> 外文期刊>The Journal of Experimental Biology >TTX-sensitive and TTX-insensitive control of spontaneous gut motility in the developing zebrafish (Danio rerio) larvae
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TTX-sensitive and TTX-insensitive control of spontaneous gut motility in the developing zebrafish (Danio rerio) larvae

机译：TTX敏感和TTX不敏感的发展中国家斑马鱼（达尼奥里奥）幼虫的自发肠道运动的控制。

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Spontaneous regular gut motility in zebrafish begins around 4 days post fertilisation (d.p.f.) and is modulated by release of acetylcholine and nitric oxide. The role of intrinsic or extrinsic innervation for initiating and propagating the spontaneous contractions, however, is not well understood. By creating spatiotemporal maps, we could examine spontaneous motility patterns in zebrafish larvae in vivo at 4 and 7 d.p.f. in more detail. Tetrodotoxin (TTX) was added to elucidate the importance of nervous control. Anterograde and retrograde contraction waves originated in the same region, just posterior to the intestinal bulb. This area correlates well with the distribution of Hu (human neuronal protein C/D)-immunoreactive nerve cell bodies. Whereas numerous immunoreactive nerve cells were present in the mid and distal intestine at both 4 and 7 d.p.f., fewer cells were seen anterior to the origin of contractions. The overall frequency of contractions (1.16+/-0.15 cycles min(-1), N=14 at 4 d.p.f.; 1.05+/-0.09 cycles min(-1), N=13 at 7 d.p.f.) and the interval between individual anterograde contraction waves (54.8+/-7.9 s at 4 d.p.f., N=14; 56.9+/-4.4 s, N=13 at 7 d.p.f.) did not differ between the two stages but the properties of the contractions were altered. The distance travelled by each wave increased from 591.0+/-43.8 microm at 4 d.p.f. (N=14) to 719.9+/-33.2 microm at 7 d.p.f. (N=13). By contrast, the velocity decreased from 4 d.p.f. (49.5+/-5.5 microm s(-1), N=12) to 7 d.p.f. (27.8+/-3.6 microm s(-1), N=13). At 4 d.p.f., TTX did not affect any of the parameters whereas at 7 d.p.f. anterograde frequency (control 1.07+/-0.12 cycles min(-1), N=8; TTX 0.55+/-0.13 cycles min(-1), N=8) and distance travelled (control 685.1+/-45.9 microm, N=8; TTX 318.7+/-88.7 microm, N=6) were decreased. In conclusion, enteric or extrinsic innervation does not seem to be necessary to initiate spontaneous contractions of the gut in zebrafish larvae. However, later in development, nerves have an increasingly important role as modulators of intestinal activity.
机译：受精后（d.p.f.）约4天，斑马鱼的自发性肠蠕动开始，并受乙酰胆碱和一氧化氮释放的调节。然而，内源性或外源性神经支配对于启动和传播自发性收缩的作用尚不十分清楚。通过创建时空图，我们可以在4和7 d.p.f的体内检查斑马鱼幼虫的自发运动模式。更详细地。添加了河豚毒素（TTX）来阐明神经控制的重要性。顺行和逆行收缩波起源于同一区域，仅在小肠球的后方。该区域与Hu（人类神经元蛋白C / D）免疫反应性神经细胞体的分布密切相关。尽管在d.p.f. 4和7 d。时在中肠和末梢肠中存在大量的免疫反应性神经细胞，但是在收缩起点之前看到的细胞较少。收缩的总频率（1.16 +/- 0.15周期min（-1），4 dpf时N = 14; 1.05 +/- 0.09周期min（-1），7 dpf时N = 13）和各个顺行之间的间隔收缩波（4 dpf时为54.8 +/- 7.9 s，N = 14; 7 dpf时为56.9 +/- 4.4 s，N = 13）在两个阶段之间没有差异，但是收缩的性质发生了变化。每波传播的距离在4 d.p.f时从591.0 +/- 43.8微米增加。 （N = 14）至7 d.p.f的719.9 +/- 33.2微米。 （N ＝ 13）。相比之下，速度从4 d.p.f.下降。 （49.5 +/- 5.5微米s（-1），N = 12）至7 d.p.f. （27.8 +/- 3.6微米s（-1），N ＝ 13）。在下午4时，TTX不会影响任何参数，而在晚上7时。顺行频率（对照1.07 +/- 0.12周期min（-1），N = 8; TTX 0.55 +/- 0.13周期min（-1），N = 8）和行进距离（对照685.1 +/- 45.9 microm，N ＝ 8; TTX 318.7 +/- 88.7微米，N ＝ 6）降低。总之，对于斑马鱼幼虫的肠道自发收缩，肠或外在神经支配似乎不是必需的。但是，在后来的发展中，神经作为肠道活性调节剂的作用越来越重要。

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