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首页> 外文期刊>Trends in Plant Science >A kaleidoscopic view of the Arabidopsis core cell cycle interactome
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A kaleidoscopic view of the Arabidopsis core cell cycle interactome

机译:拟南芥核心细胞周期相互作用组的万花筒视图

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摘要

Although protein protein interaction (PPI) networks have been shown to offer a systems-wide view of cellular processes, only a few plant PPI maps are available. Recently, the core cell cycle of Arabidopsis thaliana has been analyzed by three independent PPI technologies, including yeast two-hybrid systems, bimolecular fluorescence complementation and tandem affinity purification. Here, we merge the three interactomes with literature-curated and computationally predicted interactions, paving the way for a comprehensive picture of the plant core cell cycle machinery. Platform-specific interactions unveil the strengths and weaknesses of each detection method and give insights into the nature of the interactions among cell cycle proteins. Moreover, comparison of the obtained data reveals that a complete interactome can only be obtained when multiple techniques are applied in parallel.
机译:尽管已显示蛋白质相互作用(PPI)网络可提供系统范围的细胞过程视图,但只有少数植物PPI图可用。最近,拟南芥的核心细胞周期已经通过三种独立的PPI技术进行了分析,包括酵母双杂交系统,双分子荧光互补和串联亲和纯化。在这里,我们将三个相互作用组与文献策划和计算预测的相互作用进行合并,从而为全面了解植物核心细胞周期机制铺平了道路。特定于平台的相互作用揭示了每种检测方法的优缺点,并深入了解了细胞周期蛋白之间相互作用的性质。此外,所获得数据的比较表明,只有同时应用多种技术才能获得完整的相互作用组。

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