Agrobacterium-mediated plant transformation with large DNA fragments

Daisuke Shibata; Yao-Guang Liu

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Agrobacterium-mediated plant transformation with large DNA fragments

机译：农杆菌介导的具有大DNA片段的植物转化

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Cosmid vectors such as pOCA18 (Ref. 1) and pLZO3 (Ref. 2) have been developed that can transform plants. However, the low cloning capacity of these vectors limits their usefulness. Recently, two research groups have developed vectors for the transformation of large DNA fragments into plants. These vectors are designed to have the characteristics of both bacterial artificial chromosomes (BAVs) and binary vectors for Agrobacterium-mediated transformation.

机译：已开发出可以转化植物的粘粒载体，如pOCA18（参考文献1）和pLZO3（参考文献2）。但是，这些载体的低克隆能力限制了它们的实用性。最近，两个研究小组开发了用于将大的DNA片段转化为植物的载体。这些载体被设计为具有细菌人工染色体（BAV）和用于农杆菌介导的转化的二元载体的特征。

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《Trends in Plant Science》 |2000年第8期|共4页
作者
Daisuke Shibata; Yao-Guang Liu;
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原文格式 PDF
正文语种 eng
中图分类植物学;
关键词
agrobacterium; plant transformation; DNA;

机译：农杆菌;植物转化;DNA;

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专利

1. Agrobacterium-mediated transformation of plants: Transfer of vector DNA fragments in the plant genome [J] . Permyakova NV, Shumnyi VK, Deineko EV Russian journal of genetics . 2009,第3期

机译：农杆菌介导的植物转化：载体基因组片段在植物基因组中的转移
2. Transgenic plants from fragmented shoot tips of apple (Malus baccata (L.) Borkhausen) via agrobacterium-mediated transformation [J] . Wu Yongjie, Li Yunhe, Wu Yaqin, Scientia horticulturae . 2011,第4期

机译：通过农杆菌介导的转化从苹果（Malus baccata（L.）Borkhausen）的嫩芽尖端中转基因植物
3. Agrobacterium-Mediated Transformation of Large DNA Fragments Using a BIBAC Vector System in Rice [J] . Ruifeng He, Jin Pan, Lili Zhu, Plant Molecular Biology Reporter . 2010,第4期

机译：利用BIBAC载体系统在水稻中农杆菌介导的大DNA片段转化
4. New method for obtaining transgenic papaya plants by Agrobacterium-mediated transformation of somatic embryos [C] . ZHENTU YING, XIA YU, MICHAEL J. DAVIS Annual Meeting of the Florida State Horticultural Society . 2000

机译：用农杆菌介导的体细胞胚胎转化获得转基因木瓜植物的新方法
5. Agrobacterium-mediated transformation of Basmati rice and system development for transformation of wheat via multiple meristem bombardment. [D] . Ahmad, Anwaar. 2003

机译：农杆菌介导的印度香米的转化和通过多次分生组织轰击转化小麦的系统开发。
6. Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning [O] . Yao-Guang Liu, Yumiko Shirano, Hidehiro Fukaki, 1999

机译：具有转化能力的人工染色体载体对具有大基因组DNA片段的植物突变体的补充可加速位置克隆
7. Development of Agrobacterium-Mediated Transformation of Pear (Pyrus communis L.) with Cotyledon Explants and Production of Transgenic Pears Using ACC Oxidase cDNA. [O] . Mei GAO, Hideki MURAYAMA, Narumi MATSUDA, 2002

机译：用CATELEDON外植体的梨（Pyrus Communis L.）的梨（Pyrus Communis L.）的转化的研制使用ACC氧化酶cDNA的转基因梨生产。

Agrobacterium-mediated plant transformation with large DNA fragments

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