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DNA-mediated vaccination against infectious bursal disease in chickens

机译：DNA介导的鸡传染性法氏囊病疫苗

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摘要

The objective of the present study was to investigate the feasibility of a DNA vaccine to protect chickens against infectious bursal disease virus (IBDV) infection, A plasmid DNA carrying VP2, VP4, and VP3 genes of the standard challenge (STC) strain of IBDV was constructed and designated as pCR3.1-VP243-STC. One-day-old chickens were intramuscularly injected with the plasmid pCR3.1-VP243-STC once (group D1). twice (group D2), or three times (group D3) at weekly intervals. Chickens at 3 weeks old were orally inoculated with IBDV strain STC and observed for 10 days after challenge. Immunization twice (group D2) or three times (group 133) with the plasmid pCR3.1-VP243-STC conferred protection for 50-100 or 80-100% of chickens, respectively, as evidenced by the absence of clinical signs, mortality. and bursal atrophy. Although chickens vaccinated once (group D1) with the plasmid pCR3.1-VP243-STC did not have clinical signs. they exhibited varying degree of bursal atrophy after challenge. Enzyme-linked immunosorbent assay (ELISA) antibody titers in chickens protected by the plasmid pCR3.1-VP243-STC were significantly lower (P < 0.05) than those not protected 10 days after challenge. IBDV antigen was not detected in the bursae of chickens that were protected by receiving the plasmid pCR3.1-VP243-STC twice or three times. The results indicate that the constructed plasmid pCR3.1-VP243-STC as a DNA vaccine provided efficacious protection for chickens against IBDV infection.
机译：本研究的目的是研究保护家禽免受传染性法氏囊病病毒（IBDV）感染的DNA疫苗的可行性，带有IBDV标准攻毒（STC）株VP2，VP4和VP3基因的质粒DNA为构建并命名为pCR3.1-VP243-STC。给一天大的鸡肌肉注射质粒pCR3.1-VP243-STC一次（D1组）。每周两次（D2组）或三次（D3组）。给三周大的鸡口服IBDV毒株STC接种，攻击后观察10天。质粒pCR3.1-VP243-STC免疫两次（D2组）或三次（133组）分别为50-100％或80-100％的鸡提供了保护，如无临床体征，死亡率所证明。和法氏囊萎缩。尽管用质粒pCR3.1-VP243-STC接种过一次的鸡（D1组）没有临床症状。攻击后它们表现出不同程度的滑囊萎缩。攻击后10天，受质粒pCR3.1-VP243-STC保护的鸡的酶联免疫吸附测定（ELISA）抗体效价显着降低（P <0.05）。在通过两次或三次接受质粒pCR3.1-VP243-STC保护的鸡的法氏囊中未检测到IBDV抗原。结果表明，构建的质粒pCR3.1-VP243-STC作为DNA疫苗为鸡抗IBDV感染提供了有效的保护。

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