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首页> 外文期刊>Vaccine >Candidate peptide-vaccines induced immunity against CSFV and identified sequential neutralizing determinants in antigenic domain A of glycoprotein E2
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Candidate peptide-vaccines induced immunity against CSFV and identified sequential neutralizing determinants in antigenic domain A of glycoprotein E2

机译：候选肽疫苗诱导针对CSFV的免疫力，并在糖蛋白E2的抗原域A中确定了顺序中和决定子

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摘要

Antigenic domain A is a highly conserved unit on envelope protein E2 of classical swine fever virus (CSFV). It was found that mutant E2 containing only unit A, with the unit BC deleted, provided immunized pigs with complete protection against the lethal challenge. In this study, six overlapping peptides (A1-A6) covering this unit were synthesized and conjugated to bovine serum albumin (BSA). Two candidate multi-peptide-vaccines (MPVs) using aluminum adjuvant successfully induced potent immunity against CSFV in pigs. Although both candidate MPVs failed to provide complete protection, they showed better protective activity than that induced by C-strain. Subsequently, neutralizing epitopes in unit A were identified using a panel of peptide-vaccines (PVs). Six candidate peptide-vaccines (PV-An, n=1-6) were separately given to six groups of pigs. Among these candidates, PV-A2 and PV-A6 exhibited the most potent protective activity, while the other four showed weaker or almost no effects. Moreover, the polyclonal antibodies induced by PV-A2 and PV-A6 were capable of neutralizing C-strain virus at the dilution 1:16 in vitro. Thus, two principal sequential neutralizing determinants covered by peptide A2 (aa792-814) and A6 (aa844-865) were demonstrated to exist in the antigenic domain A, and can be recruited in developing new effective "marker vaccine" against CSFV.
机译：抗原域A是经典猪瘟病毒（CSFV）包膜蛋白E2上高度保守的单元。发现仅包含A单元而BC单元缺失的突变体E2为免疫猪提供了对致命攻击的完全保护。在这项研究中，覆盖该单元的六个重叠肽（A1-A6）被合成并与牛血清白蛋白（BSA）偶联。使用铝佐剂的两种候选多肽疫苗（MPV）成功诱导了猪对CSFV的强力免疫。尽管两种候选MPV都不能提供完全的保护，但它们显示出比C株诱导的更好的保护活性。随后，使用一组肽疫苗（PVs）鉴定单元A中的中和表位。将六种候选肽疫苗（PV-An，n = 1-6）分别给予六组猪。在这些候选物中，PV-A2和PV-A6表现出最强的保护活性，而其他四个表现出较弱或几乎没有作用。而且，由PV-A2和PV-A6诱导的多克隆抗体能够在体外以1:16的稀释度中和C株病毒。因此，证明了由肽A2（aa792-814）和A6（aa844-865）覆盖的两个主要的顺序中和决定簇存在于抗原域A中，可以在开发针对CSFV的新的有效“标志疫苗”时被招募。

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