Cryopreservation of Plant Germplasm: A Review (Scientific Review)

Rida A. Shibli; S.S. Al-Ababneh; M.A.L. Smith

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Cryopreservation of Plant Germplasm: A Review (Scientific Review)

机译：植物种质的超低温保存：综述（科学综述）

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Cryopreservation has been adopted to store base collections of plant material in liquid N_2 in order to ensure safe, and cost-effective long-term conservation of plant genetic resources. The Cryopreservation of plant material has been accomplished bymeans of several techniques, including two-step freezing, vitrification, encapsulation-dehydration, and encapsulation-vitrification. Cryopreservation must avoid the formation of ice crystals inside cells during immersion in liquid N_2 which can be achieved by lowering cell water content prior to rapid cooling. Treatments that result in low cell water content are key factors for successful Cryopreservation. Several pretreatments, including cold acclimation, exposure to abscistc acid (ABA), immersion in concentrated sugar solution or osmoticums, and air dehydration have been considered to enhance the cryopreser vability of plant material. Plant Cryopreservation protocols must include the establishment of a reliable recovery and regrowth procedures afterCryopreservation.

机译：为了确保安全，经济高效地长期保存植物遗传资源，已采用冷冻保存法将植物材料的基本集合存储在液态N_2中。植物材料的超低温保存已通过多种技术手段完成，包括两步冷冻，玻璃化，包囊脱水和包囊玻璃化。冷冻保存必须避免在浸入液体N_2期间细胞内部形成冰晶，这可以通过在快速冷却之前降低细胞水含量来实现。导致细胞含水量低的处理方法是成功进行冷冻保存的关键因素。已经考虑了几种预处理，包括冷驯化，暴露于脱落酸（ABA），浸入浓糖溶液或渗透剂中以及空气脱水以增强植物材料的低温破碎机能力。植物低温保存规程必须包括在低温保存后建立可靠的恢复和再生程序。

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《Dirasat》 |2004年第1期|共14页
作者
Rida A. Shibli; S.S. Al-Ababneh; M.A.L. Smith;
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原文格式 PDF
正文语种 eng
中图分类农业科学;
关键词
Biodiversity; Cryopreservation; encapsulation-dehydration; encapsulation-vitrification; plant genetic resources; two-step freezing; vitrification;

机译：生物多样性;低温保存;包囊脱水;包囊玻璃化;植物遗传资源;两步冷冻;玻璃化;

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7. Scientific journal «Plant and Soil Science» Font Size Make font size smaller Make font size default Make font size larger Language Select Language User Username Password Remember me Article Tools Print this article Indexing metadata How to cite item Finding References Email this article (Login required) Email the author (Login required) About The Authors YE. Krestʹyaninov National University of Life and Enviromental Sciences of Ukraine L. Yermakova National University of Life and Enviromental Sciences of Ukraine T. Antal National University of Life and Enviromental Sciences of Ukraine Social networks Information For Readers For Authors For Librarians Author Fees This journal charges the following author fees. Publication of one page: 50.00 (UAH) The fee include those of the journal’s publishing, online hosting and archiving. The ability of authors to pay the fee does not influence the peer review process. No fee can be paid prior to the final positive decision of the reviewers and the editor in charge, regarding the article proposed to be evaluated in order to be published. Depending upon each particular case, the fee can be covered by the journal edition. Details Recipient: National University of Life and Environmental Sciences of Ukraine Address: Heroyiv Oborony st., 15, Kyiv-03041, Ukraine. Current account number 31254247216289 Bank: State Treasury Service of Ukraine, Kyiv Bank code 820172 Certificate of VAT №100155865 Payment: In an article in scientific journal "Plant and Soil Science" Personal Account 18.02.06.06.01 Tel .: +38 044 527 87 20 Email: nti_dep@nubip.edu.ua Example of bibliographic description The list of journals included in scientometric databases: - Scopus (Uкraine, Belarus, Poland, Russia); - Іndex Copernicus; - Web of Sciense (humanities, natural sciences, social sciences); - РІНЦ. Search algorithm and calculation scientometric indicator: - Scopus; - Publish or Perish; - Google Scholar; - SNIP-іndex journal. Home About Login Register Search Current Archives Statistics Reminder for authors Editorial Board Home > Vol 10, No 1 (2019) > Krestʹyaninov Formation of corn grain yield and quality depending on micronutrients topdressing under conditions of Left bank Forest Steppe [O] . YE. Krestʹyaninov, L. Yermakova, T. Antal 2019

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Cryopreservation of Plant Germplasm: A Review (Scientific Review)

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