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首页> 外文期刊>Journal de mycologie medicale >Influence of nutrition and culturing conditions for optimum growth and antimicrobial metabolite production by Streptomyces sp. 201
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Influence of nutrition and culturing conditions for optimum growth and antimicrobial metabolite production by Streptomyces sp. 201

机译：营养和培养条件对链霉菌最佳生长和抗菌代谢产物产生的影响。 201

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Objective. - To evaluate the effects of certain nutrients and influence of environmental parameters on growth and production of bioactive metabolite by Streptomyces sp. 201 in submerge culture.Material and methods. - Disc diffusion method was employed to study the effect of different culture media, various carbon and nitrogen sources, inoculum size, temperature, pH and incubation period on the production of bioactive metabolite in flask cultures by Streptomyces sp. 201. Growth was determined as dry mycelial weight in a fixed volume of culture broth. Results. - Mannitol and asparagine were found to be the most suitable carbon and nitrogen sources respectively, for optimum production of growth and bioactive metabolite by the isolate. Optimum production of bioactive metabolite required mannitol in the cultivation medium with a concentration of 1.5 g/l. On the other hand, the highest antibiotic production was obtained in a cultivation medium containing 0.9 g/l of asparagine. Maximum production of bioactive metabolite in a synthetic medium was found on the sixth day of incubation. Furthermore, maintaining the incubation temperature at 30degC, pH 7.5 and the inoculum size of 2 x 10~9 spores per millilitre under shaking condition was found to be optimum for maximum antimicrobial activity.Conclusion. - The production of antimicrobial metabolite could be optimized in culturing conditions of Streptomyces sp. 201.
机译：目的。 -评估某些营养素和环境参数对链霉菌属菌生长和生产生物活性代谢物的影响。水下文化中的201.材料和方法。 -采用圆盘扩散法研究了不同培养基，碳源和氮源，接种量，温度，pH值和孵育时间对链霉菌培养的烧瓶中生物活性代谢产物产生的影响。 201.生长确定为固定体积的培养液中的干菌丝重。结果。 -发现甘露醇和天冬酰胺分别是最合适的碳源和氮源，可通过分离物最佳地产生生长物和生物活性代谢产物。为了最佳地生产生物活性代谢产物，需要在培养基中添加浓度为1.5 g / l的甘露醇。另一方面，在含有0.9g / l天冬酰胺的培养基中获得最高的抗生素产量。在培养的第六天，发现在合成培养基中生物活性代谢物的最大产量。此外，发现在摇动条件下将孵育温度保持在30°C，pH 7.5和接种物大小为每毫升2 x 10〜9个孢子是最佳的最大抗菌活性。结论。 -可以在链霉菌种的培养条件下优化抗菌代谢产物的产生。 201。

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