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首页> 外文期刊>Journal of Agricultural and Food Chemistry >STRIGA HERMONTHICA (DEL) BENTH GERMINATION STIMULANT(S) FROM MENISPERMUM DAURICUM (DC) ROOT CULTURE
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STRIGA HERMONTHICA (DEL) BENTH GERMINATION STIMULANT(S) FROM MENISPERMUM DAURICUM (DC) ROOT CULTURE

机译：稻草（MINISPERMUM DAURICUM（DC）根培养物）的STRIGA HERMONTHICA（DEL）底芽萌发剂

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摘要

Germination of Striga hermonthica, an important root parasitic weed on cereals, is induced by stimulants exuded by roots of host and some nonhost plants. Efforts to isolate active compounds have focused on root exudates from intact plants. In this paper, a much easier and less laborious novel approach, based on root cultures, is described. Menispermum dauricum roots cultured in a modified B5 medium produced and exuded into the medium compounds with activity comparable to that of the synthetic strigol analogue GR24. The active components stimulate germination by induction of ethylene biosynthesis. Their accumulation in roots and culture medium, as indicated by activity, was at a maximum 6-8 weeks after subculturing and was positively correlated with root growth (r = 0.76-0.92). HPLC and TLC of the culture filtrate revealed at least two or three compounds, none of which had chromatographic properties consistent with those of strigol. The results indicate the potentials of root cultures as a possible source of novel Striga germination stimulants.
机译：寄主植物和一些非寄主植物的根系分泌出的刺激物诱导了Striga hermonthica（一种重要的谷物寄生杂草）的发芽。分离活性化合物的努力集中在完整植物的根系分泌物上。在本文中，描述了一种基于根文化的更加轻松，省力的新颖方法。在改良的B5培养基中培养的半月板草根，产生并渗出到具有与合成strigol类似物GR24类似活性的培养基化合物中。活性成分通过诱导乙烯生物合成来刺激发芽。如活性所示，它们在根和培养基中的积累最多在继代培养后6-8周，并且与根生长呈正相关（r = 0.76-0.92）。培养滤液的HPLC和TLC显示至少两种或三种化合物，没有一种具有与strigol一致的色谱性质。结果表明，根系培养物可能是新型Striga发芽刺激物的来源。

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