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首页> 外文期刊>Journal of applied biological chemistry >Overexpression of Gene Encoding Tonoplast Intrinsic Aquaporin Promotes Urea Transport in Arabidopsis
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Overexpression of Gene Encoding Tonoplast Intrinsic Aquaporin Promotes Urea Transport in Arabidopsis

机译：编码液泡膜内在水通道蛋白的基因的过表达促进拟南芥中的尿素转运。

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Complementation assay of the urea uptake-defective yeast mutants led to the identification of the Arabidopsis AtTIP4;l gene encoding the aquaporin. However, its physiological functions still remain elusive. In the present study, histochemical and genetic analyses were performed to understand the physiological roles of AtTIP4;l in urea uptake. The AtTIP4;l product was detectible in the roots, but not hi the leaves, the stem, and the flower. Its promoter allowed the expression of the beta-glucuronidasereporter gene in the roots and the apical meristem in Arabidopsis. The AtTIP4;l products were induced under nitrogen-deficient conditions. To investigate the role of the tonoplast intrinsic protein in urea transport and developments, Arabidopsis with the loss- and the gain-of-function mutations by T-DNA insertion in AtTIP4;l and 35S promoter-mediated overexpression of AtTIP4;l were identified, respectively. The transfer DNA insertion and the Af77P4;7-overexpressed plants showed normal growth and development under normal or abiotic stress growth conditions. The urea-uptake studies using ~(14)C-labeled urea revealed higher accumulation of urea in the AtTTP4-overexpressed plants. These results provide evidence that overexpression of AtTIP4;l leads to theincrease in the urea-uptake rate in plants without detectable defects to the growth and development.
机译：尿素摄取缺陷型酵母突变体的互补分析导致鉴定了编码水通道蛋白的拟南芥AtTIP4; 1基因。但是，其生理功能仍然难以捉摸。在本研究中，进行了组织化学和遗传分析以了解AtTIP4; 1在尿素吸收中的生理作用。 AtTIP4; 1产物可在根部检出，但在叶，茎和花中未检出。它的启动子允许在根中表达β-葡糖醛酸糖苷酶报告基因和在拟南芥中的顶端分生组织。在氮缺乏的条件下诱导AtTIP4.1产物。为了研究液泡膜内在蛋白在尿素运输和发育中的作用，鉴定了通过在AtTIP4; 1和35S启动子介导的AtTIP4; 1中通过T-DNA插入而丧失功能和获得功能突变的拟南芥，分别。在正常或非生物胁迫的生长条件下，转移DNA的插入和Af77P4; 7过表达的植物显示出正常的生长发育。使用〜（14）C标记的尿素进行的尿素吸收研究表明，AtTTP4过表达的植物中尿素的积累更高。这些结果提供了证据，即AtTIP4; 1的过度表达导致植物中尿素吸收速率的增加，而对生长和发育没有可检测的缺陷。

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