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首页> 外文期刊>Journal of applied biological chemistry >Screening of Hemicellulose Oligosaccharides and Preparation of the Recipe for Modified MRS Medium by the Replacement of Carbon Source
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Screening of Hemicellulose Oligosaccharides and Preparation of the Recipe for Modified MRS Medium by the Replacement of Carbon Source

机译：半纤维素低聚糖的筛选及碳源替代制备MRS培养基的配方

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Purification and some properties of Xylogone sphaerospora beta-mannanase were reported previous paper. Locust bean gum galactomannan was hydrolyzed by the purified beta-mannanase, and then the hydrolysates was separated by activated carbon column chromatography. The main hydroly-sates were composed of D.P. (Degree of Polymerization) 4 and 6 galactosyl mannooligosaccharides. For elucidate the structure of D.P 4 and 6 galactosyl mannooligosaccharides, sequential enzymatic action was performed. D.P 4 and ,6 were identified as Gal~2Man_3 (6~2-mono-omicron-alpha-D-galactopyranosyl-4-omicron-beta-D-mannotriose) and Gal~2Man_5 (6~2-mono-omicrom-alpha-D-galacto- pyranosyl-4-omicron-beta-D-mannopentaose). To investigate the effects of locust bean gum galactosyl mannooligosaccharides on in vitro growth of Bifidobacterium longum, B. bifidum, B. infantis, B. adolescentis, B. animalis, B. auglutum and B. breve. Bifidobacterium spp. were cultivated individually on the modified-MRS medium containing carbon sourcesuch as D.P. 4 and D.P. 6 galactosyl mannooligosaccharides, respectively. B. longum and B. bifidum grew up 10-fold and 6.6-fold more effectively by the treatment of D.P. 6 galactosyl mannooligosaccharides, compared to those of standard MRS medium. Especially, D.P. 6 was more effective than D.P. 4 galactosyl mannooligosaccharide on the growth of Bifidobacterium spp.
机译：先前报道了木酮球菌β-甘露聚糖酶的纯化和一些性质。用纯化的β-甘露聚糖酶水解刺槐豆胶半乳甘露聚糖，然后通过活性炭柱色谱法分离水解产物。主要的水解产物由D.P. （聚合度）4和6个半乳糖基甘露寡糖。为了阐明D.P 4和6半乳糖甘露寡糖的结构，进行了顺序的酶促作用。 DP 4和，6被确定为Gal〜2Man_3（6〜2-单-微米-α-D-半乳糖吡喃糖基-4-omicron-β-D-甘露三糖）和Gal〜2Man_5（6〜2-单-微米-α -D-半乳糖-吡喃糖基-4-o-β-D-甘露戊糖）。研究刺槐豆胶半乳糖甘露寡糖对长双歧杆菌，双歧杆菌，婴儿双歧杆菌，青春双歧杆菌，动物双歧杆菌，金葡菌和短双歧杆菌体外生长的影响。双歧杆菌属在改良的含碳源D.P. 4和D.P.分别为6个半乳糖甘露寡糖。通过D.P.的处理，长双歧杆菌和双歧双歧杆菌的生长效率分别提高了10倍和6.6倍。与标准MRS培养基相比，含有6个半乳糖甘露寡糖。特别是D.P. 6比D.P.更有效4.半乳糖甘露寡糖对双歧杆菌属的生长。

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