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首页> 外文期刊>Journal of Biotechnology >Enhanced peroxidase production by suspension culture of carrot compact callus aggregates.
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Enhanced peroxidase production by suspension culture of carrot compact callus aggregates.

机译:通过悬浮培养胡萝卜紧密的愈伤组织聚集体来增强过氧化物酶的产生。

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摘要

A suspension culture was established from compact callus aggregates (CCA) of carrots, consisting of yellow, spherical cellular clumps displaying tissue differentiation. CCA cultures produced high amounts of peroxidase (E.C. 1.11.1.7; 5-14.5 U g-1 fresh weight). Peroxidase was detected in trace amounts in dispersed carrot cell cultures. Kinetics analysis showed that CCA grew quickly for 4-12 days with a specific growth rate of 0.2 day-1, while the peroxidase activity increased sharply after day 12.Fungal elicitors induced peroxidase synthesis. Peroxidase activity of 54 U g-1 fresh weight after addition of Aspergillus niger on day 16 (to produce a final concentration in the medium of 50 mg/litre).
机译:从胡萝卜的紧凑愈伤组织聚集体(CCA)建立悬浮培养物,该聚集体由黄色的球形细胞团块组成,表现出组织分化。 CCA培养物产生大量的过氧化物酶(E.C. 1.11.1.7; 5-14.5 U g-1鲜重)。在分散的胡萝卜细胞培养物中检测到痕量过氧化物酶。动力学分析表明,CCA快速生长4-12天,比生长速率为0.2 day-1,而过氧化物酶活性在第12天后急剧增加。真菌诱导剂诱导过氧化物酶合成。在第16天加入黑曲霉后过氧化物酶活性为54 U g-1新鲜重量(在培养基中的终浓度为50 mg / L)。

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