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首页> 外文期刊>Journal of Biotechnology >Effect of fermentation temperature on validamycin A production by Streptomyces hygroscopicus 5008
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Effect of fermentation temperature on validamycin A production by Streptomyces hygroscopicus 5008

机译：发酵温度对吸水链霉菌5008生产有效霉素A的影响

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摘要

Validamycin A (VAL-A), produced by Streptomyces hygroscopicus, is an important anti-fungal agro-antibiotic. In this work, the effect of fermentation temperature on VAL-A biosynthesis by S. hygroscopicus 5008 was investigated between 28pC and 42pC, and an interesting threshold of temperature for VAL-A biosynthesis was found between 35pC and 37pC. At a relatively higher temperature, a much higher VAL-A productivity was obtained together with faster protein synthesis and sugar consumption. Transcriptional analysis of samples from early, middle and late stages of fermentation at various temperatures demonstrated that three operons, valABC, valKLMN and valG, for all eight necessary structure genes, were dramatically promoted when temperature reached the threshold. Activities of both glucose-6-phosphate dehydrogenase (G6PDH) of pentose-phosphate pathway and ValG of VAL-A biosynthesis were also enhanced at a higher cultivation temperature. The interesting temperature effect with a 2pC threshold shift from 35pC to 37pC on the antibiotic biosynthesis was understood to be related to the gene transcriptional levels and key enzyme activities.
机译：吸水链霉菌生产的Validamycin A（VAL-A）是一种重要的抗真菌农业抗生素。在这项工作中，研究了发酵温度对吸水链霉菌5008在VAL-A生物合成中的影响，温度介于28pC和42pC之间，在35pC和37pC之间发现了一个有趣的VAL-A生物合成温度阈值。在相对较高的温度下，可获得更高的VAL-A生产率以及更快的蛋白质合成和糖消耗。在不同温度下对发酵的早期，中期和后期进行的样品的转录分析表明，温度达到阈值时，对于八个必需的结构基因，三个操纵子valABC，valKLMN和valG都得到了显着促进。在较高的培养温度下，戊糖磷酸途径的葡萄糖6-磷酸脱氢酶（G6PDH）和VAL-A生物合成的ValG的活性也均增强。从35pC到37pC的2pC阈值偏移对抗生素生物合成产生了有趣的温度效应，这被认为与基因转录水平和关键酶活性有关。

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