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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Adsorption-desorption of proteins on phospholipid polymer surfaces evaluated by dynamic contact angle measurement.
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Adsorption-desorption of proteins on phospholipid polymer surfaces evaluated by dynamic contact angle measurement.

机译：通过动态接触角测量评估蛋白质在磷脂聚合物表面上的吸附-解吸。

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摘要

Adsorption-desorption of plasma protein on various polymer membranes was evaluated by a dynamic contact angle (DCA) measurement using the Wilhelmy plate method. Poly(ethylene terephthalate) (PET) was used as a substrate membrane; we examined this membrane coated with hydrophilic polymers such as poly[2-methacryloyloxethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)] or poly[2-hydroxyethyl methacrylate (HEMA)]. Although the zeta-potential of the PET membrane was negative, the coating with poly(MPC-co-BMA) induced increase of value to nearly zero. The DCA loops observed on the polymer membranes after protein adsorption were unity and hysteresis of the loop was reduced. In the cases of protein adsorbed on both the PET and the poly(HEMA) membranes, the shape and hysteresis of the loops were almost same during the rinsing process with a phosphate-buffered solution (PBS). However, the hysteresis of the DCA loops that appeared on the protein-adsorbed poly(MPC-co-BMA) membrane became large during therinsing process with the PBS, and the shape of the DCA loop returned to its non-protein-adsorbed state. Therefore, proteins adsorbed on poly(MPC-co-BMA) could desorb more readily than those on PET and poly(HEMA) because of the weak interaction between poly(MPC-co-BMA) and the proteins.
机译：使用Wilhelmy平板法通过动态接触角（DCA）测量来评估血浆蛋白在各种聚合物膜上的吸附-解吸。聚对苯二甲酸乙二醇酯（PET）用作基材膜;我们检查了这种涂有亲水聚合物的膜，例如聚[2-甲基丙烯酰氧乙基磷酰胆碱（MPC）-甲基丙烯酸正丁酯（BMA）]或聚[甲基丙烯酸2-羟乙酯（HEMA）]。尽管PET膜的Zeta电位为负，但使用聚（MPC-co-BMA）涂层引起的值增加到接近零。蛋白质吸附后在聚合物膜上观察到的DCA环是一体的，并且环的磁滞减小了。在蛋白质吸附在PET和聚（HEMA）膜上的情况下，在用磷酸盐缓冲液（PBS）冲洗的过程中，环的形状和滞后几乎相同。然而，在用PBS漂洗过程中，蛋白质吸附的聚（MPC-co-BMA）膜上出现的DCA环的滞后变大，并且DCA环的形状返回到其非蛋白质吸附状态。因此，由于聚（MPC-co-BMA）与蛋白质之间的相互作用较弱，因此与聚（HEMA）上的蛋白质相比，聚（MPC-co-BMA）上的蛋白质吸附更容易。

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