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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Protein adsorption on titanium surfaces and their effect on osteoblast attachment
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Protein adsorption on titanium surfaces and their effect on osteoblast attachment

机译：蛋白质在钛表面的吸附及其对成骨细胞附着的影响

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摘要

The objective of this study was to investigate the adsorption of albumin and fibronectin on titanium (Ti) surfaces and the effect of preadsorbed albumin and fibronectin on osteoblast attachment in vitro. Bovine serum albumin and bovine fibronectin were used in this study. Maximum adsorption of bovine serum albumin and fibronectin on Ti surfaces was observed to occur after 180-min incubation. In the presence of preadsorbed proteins, osteoblast attachment on Ti surfaces was observed to be enhanced compared to control Ti surfaces. However, cell attachment was affected by the types of protein adsorbed. Preadsorbed albumin was observed to have no significant effect on the amount of osteoblast cells attached. In comparison to control Ti surface and Ti surfaces preadsorbed with albumin, Ti surfaces preadsorbed with fibronectin for 15 mm was observed to significantly increase osteoblast cell attachment, whereas Ti surfaces preadsorbed with fibronectin for 180 min did not affect cell attachment. In addition, cell morphology of the attached cells on protein preadsorbed Ti surfaces was not affected by the type of protein used in this study. It was concluded from this study that the concentration of fibronection adsorbed on Ti surfaces was higher compared to albumin. In addition, it was also concluded that the concentration of fibronectin on Ti surfaces plays a role in governing cell attachment.
机译：这项研究的目的是调查白蛋白和纤连蛋白在钛（Ti）表面的吸附以及体外预先吸附的白蛋白和纤连蛋白对成骨细胞附着的影响。本研究使用牛血清白蛋白和牛纤连蛋白。孵育180分钟后，观察到牛血清白蛋白和纤连蛋白在Ti表面的最大吸附。在存在预吸附蛋白的情况下，与对照Ti表面相比，观察到Ti表面上的成骨细胞附着得以增强。但是，细胞附着受吸附蛋白类型的影响。观察到预先吸附的白蛋白对附着的成骨细胞数量没有明显影响。与对照Ti表面和白蛋白预吸附的Ti表面相比，观察到纤连蛋白预吸附15 mm的Ti表面可显着增加成骨细胞的附着，而纤连蛋白预吸附180 min的Ti表面则不影响细胞的附着。此外，本研究中使用的蛋白质类型不影响蛋白质预吸附的Ti表面上附着细胞的细胞形态。从这项研究得出的结论是，与白蛋白相比，钛表面吸附的纤维蛋白浓度更高。另外，还得出结论，Ti表面上纤连蛋白的浓度在控制细胞附着中起作用。

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