In vitro models are important investigative tools in understanding the biological processes involved in wear-particle-induced chronic inflammation and periprosthetic osteolysis. In the clinical scenario, particles are produced and delivered continuously over extended periods of time. Previously, we quantified the delivery of both polystyrene and polyethylene particles over 2- and 4-week time periods using osmotic pumps and collection tubes. In the present study, we used explanted mice femora in organ culture and showed that continuous intramedullary delivery of submicron-sized polymer particles using osmotic pumps is feasible. Furthermore, infusion of 2.60 x 10(11) particles per mL (intermediate concentration) of ultrahigh molecular weight polyethylene (UHMWPE) for 2 weeks and 8.06 x 10(11) particles per mL (high concentration) UHMWPE for 4 weeks both yielded significantly higher scores for bone loss when compared with controls in which only mouse serum was infused.
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机译：体外模型是了解磨损颗粒诱导的慢性炎症和假体周围骨溶解涉及的生物学过程的重要研究工具。在临床情况下，会在较长的时间内连续产生和输送颗粒。以前，我们使用渗透泵和收集管量化了在2周和4周时间内聚苯乙烯和聚乙烯颗粒的输送量。在本研究中，我们在器官培养中使用了离体的小鼠股骨，并证明了使用渗透泵连续髓内递送亚微米级聚合物颗粒是可行的。此外，每毫升（中等浓度）超高分子量聚乙烯（UHMWPE）注入2.60 x 10（11）颗粒持续2周，每毫升（高浓度）UHMWPE注入8.06 x 10（11）粒子持续4周，均显着提高与仅注入小鼠血清的对照组相比，骨丢失评分较高。
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