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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Cell adhesion on supported lipid bilayers
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Cell adhesion on supported lipid bilayers

机译：细胞在支持的脂质双层上的粘附

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摘要

The cell and protein repellent properties of supported phospholipid bilayer (SPB) membranes were investigated. The SPBs were prepared by vesicle adsorption on SiO_2 surfaces. The vesicles of phosphatidylcholine fuse and rupture, and form a supported bilayer covering the surface. We carried out cell culture experiments on several surfaces, including SPBs, using two types of epithelial cells to address the cell adhesional properties. The Quartz Crystal Microbalance Dissipation (QCM-D) technique was used to monitor the SPB formation and subsequent protein adsorption. Neither cell type adhered or proliferated on SiO_2 surfaces coated with SPBs, whereas both cell types adhered and proliferated on the three control surfaces of SiO_2, tissue culture glass, and TiO_2. The QCM-D measurements showed that about two orders of magnitude less mass adsorbed on a SPB surface compared to a TiO_2 surface, from serum-containing media (10 percent fetal bovine serum). The reduced adsorption on the SPB is a likely explanation for the nondetectable epithelial cell adhesion on the SPB surface. Biomembranes are therefore attractive candidate systems to achieve alternating cell-resistant and cell-interacting regions on surfaces, by including specific cell-binding proteins in the latter regions.
机译：研究了支持的磷脂双层（SPB）膜的细胞和蛋白质排斥特性。通过在SiO_2表面上进行囊泡吸附来制备SPB。磷脂酰胆碱的囊泡融合并破裂，并形成覆盖表面的支撑双层。我们使用两种类型的上皮细胞在包括SPB在内的多个表面上进行了细胞培养实验，以解决细胞粘附特性。石英晶体微平衡耗散（QCM-D）技术用于监测SPB的形成和随后的蛋白质吸附。两种细胞类型均不会在涂有SPB的SiO_2表面上粘附或增殖，而两种细胞类型均会在SiO_2，组织培养玻璃和TiO_2的三个控制表面上粘附和增殖。 QCM-D测量表明，从含血清的介质（10％胎牛血清）中，与TiO_2表面相比，SPB表面吸附的质量减少了大约两个数量级。 SPB上吸附的减少可能是SPB表面上无法检测到的上皮细胞粘附的解释。因此，生物膜是吸引人的候选系统，通过在后面的区域中包含特定的细胞结合蛋白，可以在表面上实现交替的细胞抗性和细胞相互作用区域。

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