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首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >Determination of liquiritigenin by ultra high performance liquid chromatography coupled with triple quadrupole mass spectrometry: Application to a linear pharmacokinetic study of liquiritigenin in rat plasma
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Determination of liquiritigenin by ultra high performance liquid chromatography coupled with triple quadrupole mass spectrometry: Application to a linear pharmacokinetic study of liquiritigenin in rat plasma

机译：超高效液相色谱-三重四极杆质谱联用测定脂蛋白原：在大鼠血浆中脂蛋白原的线性药代动力学研究中的应用

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摘要

A simple, sensitive and rapid ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method has been developed and validated for the quantification of liquiritigenin, a promising anti-tumor agent. Liquiritigenin and the internal standard were separated on an Agilent Extend C-18 column and eluted with a gradient mobile phase system of acetonitrile and water. The analysis was performed on a negative ionization electrospray mass spectrometer via multiple reaction monitoring (MRM). Transitions of m/z 255.0 -> 119.0 for liquiritigenin and m/z 269.0 -> 117.0 for the IS were monitored. One-step protein precipitation with acetonitrile was used to remove impurities and extract the analytes from plasma. The method had a chromatographic run time of 4.5 min and a good linearity in the range of 1-1000 ng/mL. The precision (R.S.D.) of intra-day and inter-day ranged from 4.54 to 10.65% and 5.94 to 13.81%, respectively; while the accuracy of intra-day and inter-day ranged from 104.06 to 109.28% and 94.98 to 112.05%. The recovery and stability were also within the acceptable limits. The validated method was applied to a linear pharmacokinetic study of liquiritigenin in rat plasma for the first time. (C) 2014 Published by Elsevier B.V.
机译：已经开发了一种简单，灵敏，快速的超高效液相色谱串联质谱（UHPLC-MS / MS）方法，并已验证该方法可用于定量有希望的抗肿瘤药物Liquiritigenin。在安捷伦Extend C-18色谱柱上分离Liquiritigenin和内标，并用乙腈和水的梯度流动相系统洗脱。通过多反应监测（MRM）在负离子电喷雾质谱仪上进行分析。监测了甜橙素的m / z 255.0-> 119.0和IS的m / z 269.0-> 117.0的转变。用乙腈进行一步蛋白质沉淀可去除杂质并从血浆中提取分析物。该方法的色谱运行时间为4.5分钟，线性度在1-1000 ng / mL范围内。日内和日间的精确度（R.S.D.）分别为4.54至10.65％和5.94至13.81％;日内和日间的准确度介于104.06至109.28％和94.98至112.05％之间。回收率和稳定性也在可接受的范围内。验证的方法首次应用于大鼠血浆中liquiritigenin的线性药代动力学研究。 （C）2014由Elsevier B.V.发布

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