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首页> 外文期刊>Journal of dermatological science >Proteolytic digest derived from bovine Ligamentum Nuchae stimulates deposition of new elastin-enriched matrix in cultures and transplants of human dermal fibroblasts.
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Proteolytic digest derived from bovine Ligamentum Nuchae stimulates deposition of new elastin-enriched matrix in cultures and transplants of human dermal fibroblasts.

机译：源自牛韧带的蛋白质水解消化物刺激了人类皮肤成纤维细胞的培养和移植过程中新的富含弹性蛋白的基质的沉积。

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BACKGROUND: Diverse topical products and injectable fillers used for correcting facial wrinkles induce rather short-lived effects because they target replacement of dermal collagen and hyaluronan, matrix components of limited biologic durability. OBJECTIVE: Present studies were aimed at stimulation of fully differentiated human dermal fibroblasts to resume deposition of new extracellular matrix rich of elastin, the most durable and metabolically inert component of dermal ECM. METHODS: We have created a novel proteolytic digest of bovine ligamentum nuchae (ProK-60), and tested its potential biological effect on dermal fibroblasts derived from females of different ages. Northern blots, quantitative immunohistochemistry and metabolic assays were used to assess effects of ProK-60 on proliferation and matrix production in primary cultures of dermal fibroblasts, in cultures of skin explants and after implantation of stimulated fibroblasts into the skin of athymic nude mice. RESULTS: ProK-60 increased proliferation (25-30%) of cultured dermal fibroblasts and significantly enhanced their production of new elastic fibers (>250%) and collagen fibers (100%). These effects were mostly mediated by stimulation of cellular elastin receptor. In contrast, ProK-60 inhibited production of fibronectin (-30%) and chondroitin sulfate proteoglycans (-50%). ProK-60 also activated proliferation of dermal fibroblasts, mostly derived from the stratum basale and induced deposition of elastic fibers in cultures of skin explants. Moreover, human fibroblasts pre-treated with ProK-60 produced abundant elastic fibers after their injection into the skin of athymic nude mice. CONCLUSION: The described biological effects of ProK-60, including its unique elastogenic property, encourage use of this compound in cosmetic formulations stimulating rejuvenation of aged skin.
机译：背景：用于矫正面部皱纹的各种局部用产品和可注射填充剂具有相当短暂的效果，因为它们的目标是替代皮肤胶原蛋白和透明质酸，这是生物耐久性有限的基质成分。目的：目前的研究旨在刺激完全分化的人类皮肤成纤维细胞，以恢复富含弹性蛋白的新细胞外基质的沉积，弹性蛋白是皮肤ECM中最持久和代谢惰性的成分。方法：我们创建了一种新颖的牛韧带蛋白水解酶消化物（ProK-60），并测试了其对不同年龄女性的真皮成纤维细胞的潜在生物学作用。使用Northern印迹，定量免疫组织化学和代谢分析来评估ProK-60对真皮成纤维细胞的原代培养，皮肤外植体培养以及将刺激的成纤维细胞植入无胸腺裸鼠皮肤中后增殖和基质产生的影响。结果：ProK-60增加了培养的真皮成纤维细胞的增殖（25-30％），并显着提高了其新弹性纤维（> 250％）和胶原纤维（100％）的产生。这些作用主要是通过刺激细胞弹性蛋白受体介导的。相反，ProK-60抑制了纤连蛋白（-30％）和硫酸软骨素蛋白聚糖（-50％）的产生。 ProK-60还激活了真皮成纤维细胞的增殖，这些成纤维细胞主要来源于基底层，并诱导了皮肤外植体培养物中弹性纤维的沉积。此外，用ProK-60预处理的人成纤维细胞在注射入无胸腺裸鼠皮肤后产生了丰富的弹性纤维。结论：ProK-60的上述生物学作用，包括其独特的弹性生成特性，促使该化合物在化妆品配方中使用，以刺激衰老的皮肤恢复活力。

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