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首页> 外文期刊>Journal of endotoxin research >Fever onset is linked to the appearance of lipopolysaccharide in the liver.
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Fever onset is linked to the appearance of lipopolysaccharide in the liver.

机译：发烧与肝脏中脂多糖的出现有关。

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摘要

To assess the relative contributions of different phagocytes to the febrile response of guinea pigs to intravenous (i.v.) and intraperitoneal (i.p.) bacterial endotoxic lipopolysaccharide (LPS), we injected fluorescein isothiocyanate (FITC)-labeled LPS at doses of 37.5, 75, 150, 300 and 900 microg/kg, and measured its distribution and corresponding core temperature (T(c)) changes before and at 15, 30, 60, 90, and 120 min after injection. At all times, i.v. FITC-LPS appeared as granular fluorescent patches in circulating leukocytes and hepatic macrophages; its density was proportional to dose. At all doses, the density of i.v. FITC-LPS labeling decreased from its peak 15 min after injection at a rate commensurate with its dose. Intraperitoneal FITC-LPS was also present dose- and time-dependently in peritoneal macrophages, but it appeared later and accumulated more slowly except at the highest dose. Compared with i.v. FITC-LPS, its maximal appearance was always lower in density. No labeling was found at any time in brain and kidney following any dose of i.v. or i.p. FITC-LPS injection. The initiation of T(c) rises was best correlated with the presence of FITC-LPS in liver, irrespective of its route of injection. Pretreatment with gadolinium chloride 3 days before LPS injection attenuated the febrile response and reduced FITC-LPS labels in liver. These results suggest that the Kupffer cells may be central to the initiation of the febrile response of guinea pigs to i.v. and i.p. LPS.
机译：为了评估不同吞噬细胞对豚鼠对静脉（iv）和腹膜内（ip）细菌内毒素脂多糖（LPS）的发热反应的相对贡献，我们以37.5、75、150的剂量注射了异硫氰酸荧光素标记的LPS分别为300和900 microg / kg，并在注射前和注射后15、30、60、90和120分钟测量其分布和相应的核心温度（T（c））变化。在任何时候，i.v。 FITC-LPS在循环白细胞和肝巨噬细胞中以颗粒状荧光斑的形式出现。其密度与剂量成正比。在所有剂量下，静脉注射的密度FITC-LPS标记从注射后15分钟的峰值开始以与其剂量相称的速率下降。腹膜内FITC-LPS在腹膜巨噬细胞中也呈剂量依赖性和时间依赖性，但出现时间较晚，且积累速度较慢，但​​最高剂量除外。与i.v.比较FITC-LPS，其最大外观总是密度较低。静脉内注射任何剂量后，在任何时间都未在脑和肾中发现标记。或i.p. FITC-LPS注射。无论注射途径如何，T（c）升高的开始与肝脏中FITC-LPS的存在最相关。 LPS注射前3天用氯化g预处理可减轻肝脏的发热反应并减少FITC-LPS标签。这些结果表明，Kupffer细胞对于豚鼠对i.v.的热反应的启动可能是关键的。和ip LPS。

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