...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of endotoxin research >Hemoperfusion with polymyxin B-immobilized fibers reduced the number of CD16+ CD14+ monocytes in patients with septic shock.
【24h】

Hemoperfusion with polymyxin B-immobilized fibers reduced the number of CD16+ CD14+ monocytes in patients with septic shock.

机译：败血性休克患者血液中灌注多粘菌素B固定纤维减少了CD16 + CD14 +单核细胞的数量。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: CD16+ CD14+ monocytes dramatically increase in number in patients with severe infection. Hemoperfusion with PMX-F (direct hemoperfusion with polymyxin B immobilized fibers) has been reported to be a safe and effective treatment for patients with septic shock, although the molecular mechanism that accounts for its effectiveness is still unclear. The purpose of this study was to quantify the number of CD16+ CD14+ monocytes in patients with an intra-abdominal infection and to evaluate the effects of PMX-F treatment on clinical parameters and leukocyte surface antigen expression in these patients. MATERIALS AND METHODS: Seventeen septic patients who had an intra-abdominal infection were enrolled in this study; 7 of these patients received PMX-F treatment. Peripheral blood samples were obtained immediately after admission, and were also collected from the above 7 patients before, during, and immediately after their PMX-F treatment. The expression of CD14, CD16, and Toll-like receptor (TLR)-4 on these patients' monocytes was evaluated using flow cytometry. In addition, lipopolysaccharide (LPS)-induced production of TNF-alpha and IL-1beta by these cells was measured by ELISA. RESULTS: Monocytic expression of CD16 and TLR-4 was significantly greater in septic patients than in healthy controls, and their proportion of CD16+ CD14+ monocytes was similarly elevated. LPS-induced production of TNF-alpha and IL-1beta by peripheral blood mononuclear cells (PBMCs) of septic patients was significantly reduced compared to controls. Furthermore, there was a reduction in the proportion of CD16+ CD14+ monocytes during PMX-F treatment, and in the expression of TLR-4 on monocytes after PMX-F treatment. CONCLUSIONS: These results showed that the number of peripheral blood CD16+ CD14+ monocytes and monocytic TLR-4 expression were markedly increased, and the production of pro-inflammatory cytokines in response to LPS significantly reduced in patients with sepsis. PMX-F treatment was found to be effective in reducing the number of CD16+ CD14+ monocytes and in decreasing the monocytic expression of TLR-4 in patients with septic shock.
机译：背景：患有严重感染的患者中CD16 + CD14 +单核细胞数量急剧增加。据报道，对于脓毒性休克患者，使用PMX-F进行血液灌流（使用固定化多粘菌素B的纤维直接进行血液灌流）是一种安全有效的治疗方法，尽管尚不清楚其有效性的分子机制。这项研究的目的是量化腹腔内感染患者中CD16 + CD14 +单核细胞的数量，并评估PMX-F治疗对这些患者的临床参数和白细胞表面抗原表达的影响。材料与方法：本研究招募了十七名患有腹腔内感染的败血病患者。这些患者中有7位接受了PMX-F治疗。入院后立即获得外周血样本，并在接受PMX-F治疗之前，期间和之后从上述7名患者中收集。使用流式细胞术评估了这些患者单核细胞上CD14，CD16和Toll样受体（TLR）-4的表达。另外，通过ELISA测量了这些细胞由脂多糖（LPS）诱导的TNF-α和IL-1β的产生。结果：脓毒症患者中CD16和TLR-4的单核细胞表达明显高于健康对照组，其CD16 + CD14 +单核细胞的比例也同样升高。与对照组相比，脓毒症患者外周血单核细胞（PBMC）的LPS诱导的TNF-α和IL-1beta产生显着降低。此外，在PMX-F处理期间，CD16 + CD14 +单核细胞的比例降低，并且在PMX-F处理之后，在单核细胞上TLR-4的表达降低。结论：这些结果表明败血症患者外周血CD16 + CD14 +单核细胞数量和单核细胞TLR-4表达显着增加，并且对LPS的促炎性细胞因子产生明显减少。发现在患有败血性休克的患者中，PMX-F治疗可有效减少CD16 + CD14 +单核细胞的数量并减少TLR-4的单核细胞表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号