...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >Different and overlapping functions of Arabidopsis LHT6 and AAP1 transporters in root amino acid uptake
【24h】

Different and overlapping functions of Arabidopsis LHT6 and AAP1 transporters in root amino acid uptake

机译：拟南芥LHT6和AAP1转运蛋白在根氨基酸吸收中的不同和重叠功能

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Plants acquire nitrogen in the form of amino acids from the soil, and transport proteins located in the plasma membrane of root cells are required for this process. It was found that the Arabidopsis lysine-histidine-like transporter LHT6 is expressed in root cells important for amino acid uptake, including the epidermis, root hairs, and cortex. Transport studies with lht6 mutants using high levels of amino acids demonstrated that LHT6 is in fact involved in amino acid uptake. To determine if LHT6 plays a role in nitrogen acquisition at soil amino acid concentrations, growth and uptake studies were performed with low levels of toxic amino acid analogues and radiolabelled amino acids, respectively. In addition, mutants of AAP1, another root amino acid transporter, and lht6/aap1 double mutants were examined. The results showed that LHT6 is involved in uptake of acidic amino acids, glutamine and alanine, and probably phenylalanine. LHT6 seems not to transport basic or other neutral amino acids, or, alternatively, other transporters might compensate for eliminated LHT6 function. Previous studies suggested that AAP1 only takes up amino acids at high concentrations; however, here it is demonstrated that the transporter functions in acquisition of glutamate and neutral amino acids when present at soil concentrations. When comparing the characterized root uptake systems, it appears that transporters both with overlapping substrate specificity and with preference for specific substrates are required to access the soil amino acid pool.
机译：植物从土壤中获取氨基酸形式的氮，而此过程需要位于根细胞质膜中的转运蛋白。已经发现，拟南芥类赖氨酸-组氨酸样转运蛋白LHT6在对氨基酸摄取重要的根细胞中表达，包括表皮，根毛和皮质。使用高水平氨基酸对lht6突变体的转运研究表明，LHT6实际上与氨基酸摄取有关。为了确定LHT6在土壤氨基酸浓度下是否在氮素获取中起作用，分别对低含量的有毒氨基酸类似物和放射性标记的氨基酸进行了生长和吸收研究。此外，检查了AAP1，另一个根氨基酸转运蛋白的突变体和lht6 / aap1双突变体。结果表明，LHT6与酸性氨基酸，谷氨酰胺和丙氨酸以及苯丙氨酸的摄取有关。 LHT6似乎不转运碱性或其他中性氨基酸，或者，其他转运蛋白可能补偿了已消除的LHT6功能。先前的研究表明，AAP1只吸收高浓度的氨基酸。然而，在这里证明了当存在于土壤浓度时，转运蛋白在获取谷氨酸和中性氨基酸中起作用。当比较特征性的根部吸收系统时，似乎需要转运蛋白具有重叠的底物特异性并且优先选择特定的底物才能进入土壤氨基酸库。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号