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首页> 外文期刊>Journal of Fish Biology >A molecular approach to detect hybridization between bream Abramisbrama, roach Rutlius rutilus and rudd Scardinius erythrophthalmus
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A molecular approach to detect hybridization between bream Abramisbrama, roach Rutlius rutilus and rudd Scardinius erythrophthalmus

机译：一种检测鲷鱼，蟑螂Rutius rut​​ilus和陆克Scardinius erythrophthalmus之间杂交的分子方法

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摘要

Hybridization between roach Rutilus rutilus, bream Abramis brama and rudd Scardinius erythrophthalmus was investigated using morphological and genetic analysis employing both nuclear (ITS1) and mitochondrial (cytochrome b) markers. Allele-specific amplification (ASA) reactions for both markers were developed and ITS1 sequence data for all three species are presented. Sequencing detected two ITS1 haplotypes within both roach and bream which most likely evolved in isolation and were subsequently brought together as a result of restocking by anglers. Analysis of cloned hybrid nuclear ribosomal DNA revealed evidence of recombination between parental ITS1 sequences. ASA proved to be an effective method for identifying hybrids and detected multiple ITS1 copies in fishes identified as purebred by morphological analysis. In addition this suggests post-F1 hybridization and introgression may be occurring between roach and bream, and rudd and bream, although some barriers appear to be suppressing backcrosses within the hybrid population. Analysis of the hybrid population demonstrated that hybridization has occurred in both directions.
机译：利用形态学和遗传分析，利用核（ITS1）和线粒体（细胞色素b）标记，对蟑螂Rutilus rut​​ilus，鲷Abramis brama和陆生Scardinius erythrophthalmus进行了杂交。开发了两种标记的等位基因特异性扩增（ASA）反应，并提供了所有三种物种的ITS1序列数据。测序发现蟑螂和鲷中有两种ITS1单倍型，很可能是孤立进化的，后来由于垂钓者的补给而被整合在一起。克隆的核核糖体DNA杂合体的分析揭示了亲本ITS1序列之间重组的证据。 ASA被证明是一种鉴定杂种的有效方法，并通过形态学分析在纯种鱼中检测到多个ITS1拷贝。此外，这表明蟑螂与，陆尾与之间可能发生F1后杂交和渗入，尽管某些障碍似乎抑制了杂交种群内的回交。对杂种种群的分析表明，杂种发生在两个方向。

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