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首页> 外文期刊>Journal of immunoassay >Use of dot-blot immunogold assay to identify a proliferative antigen in the initial cells of a wheat stem meristem.
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Use of dot-blot immunogold assay to identify a proliferative antigen in the initial cells of a wheat stem meristem.

机译:使用斑点印迹免疫金法鉴定小麦茎分生组织初始细胞中的增殖抗原。

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摘要

We have devised a protocol for the isolation and identification of a proliferative antigen of the initial cells of wheat stem meristems (termed PAI). We have carried out a variety of immunochemical and immunocytochemical methods, using colloidal gold (CG) complexed with monospecific antibodies to PAI as the marker for the detection of PAI. We have been able to determine the effectiveness of immunoaffinity chromatography in isolating PAI from plant tissues and have shown the advantages of CG over enzyme labels for identification of the antigen. Finally, we have obtained a purified preparation of PAI and have determined its molecular mass (approximately 83 kDa).
机译:我们设计了一种协议,用于分离和鉴定小麦干分生组织的初始细胞(称为PAI)的增殖抗原。我们已经进行了多种免疫化学和免疫细胞化学方法,使用胶体金(CG)与针对PAI的单特异性抗体复合作为检测PAI的标记。我们已经能够确定从植物组织中分离PAI的免疫亲和层析的有效性,并显示了CG优于酶标记物用于鉴定抗原的优势。最后,我们获得了纯化的PAI制剂,并确定了其分子量(约83 kDa)。

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