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首页> 外文期刊>Journal of Insect Physiology >Involvement of ERK/MAPK in regulation of diapause intensity in the false melon beetle, Atrachya menetriesi
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Involvement of ERK/MAPK in regulation of diapause intensity in the false melon beetle, Atrachya menetriesi

机译：ERK / MAPK参与调节假甲虫Atrachya menetriesi的滞育强度

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摘要

Embryonic diapause is commonly terminated by exposure to low temperature for a certain duration. Previous studies using the silkworm, Bombyx mori, showed that extracellular signal-regulated kinase (ERK), a member of the mitogen-activated protein kinase family, was activated by cold exposure and regulated diapause termination. The involvement of ERK in regulation of diapause termination was investigated in the false melon beetle, Atrachya menetriesi. Embryonic diapause of this beetle is terminated both by cold exposure and by mercury. Phospho-ERK levels remained high during the pre-diapause period but decreased after the eggs entered diapause. Exposure to 7.5 degrees C, which was effective for diapause termination, increased phospho-ERK levels, and these levels were maintained under 7.5 degrees C at least for 100d. Incubation at 25 degrees C after the eggs were kept at 7.5 degrees C for 20d, which intensified diapause, decreased the phospho-ERK level. An insufficient cold treatment, i.e., incubation at 0 degrees C for diapause termination did not activate ERK. However, incubation at 0 degrees C after cold treatment at 7.5 degrees C, which is effective for diapause termination, induced high phospho-ERK levels. Moreover, mercury treatment also increased phospho-ERK. Therefore, changes in the phospho-ERK level correlated well with diapause intensity. The results suggest that ERK plays a key role in the regulation of embryonic diapause.
机译：通常通过暴露于低温一定时间来终止胚胎滞育。以前使用家蚕（Bombyx mori）的研究表明，细胞外信号调节激酶（ERK）是有丝分裂原激活的蛋白激酶家族的成员，被冷暴露激活并调节了滞育终止。在假甜瓜甲虫Atrachya menetriesi中调查了ERK在滞育终止调控中的参与。甲虫的胚胎滞育通过冷暴露和水银终止。滞育前期，磷酸-ERK的含量仍然很高，但是在卵进入滞育期后却降低了。暴露于7.5摄氏度（这对于滞育终止有效），增加了磷酸化ERK的水平，这些水平至少在7.5摄氏度下保持100天。将卵在7.5摄氏度下放置20天后在25摄氏度下孵育，加剧了滞育，降低了磷酸化ERK水平。不足的冷处理，即在0°C下孵育以停止滞育不会激活ERK。然而，对滞育终止有效的在7.5℃下冷处理后在0℃下进行的孵育诱导了高磷酸化ERK水平。此外，汞处理也增加了磷酸化-ERK。因此，磷酸-ERK水平的变化与滞育强度密切相关。结果表明ERK在胚胎滞育的调节中起关键作用。

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