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首页> 外文期刊>Journal of Environmental Quality >Persistence of Culturable Escherichia coli Fecal Contaminants in Dairy Alpine Grassland Soils
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Persistence of Culturable Escherichia coli Fecal Contaminants in Dairy Alpine Grassland Soils

机译：乳品高寒草原土壤中可培养的大肠杆菌粪便污染物的持久性

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Our knowledge of Escherichia coli (E. coli) ecology in the field is very limited in the case of dairy alpine grassland soils. Here, our objective was to monitor field survival of E. coli in cow pats and underlying soils in four different alpine pasture units, and to determine whether the soil could constitute an environmental reservoir. E. coli was enumerated by MPN using a selective medium. E. coli survived well in cow pats (10(7) to 10(8) cells g-1 dry pat), but cow pats disappeared within about 2 mo. In each pasture unit, constant levels of E. coli (10(3) to 10(4) cells g-1 dry soil) were recovered from all topsoil (0-5 cm) samples regardless of the sampling date, that is, under the snow cover, immediately after snow melting, or during the pasture season (during and after the decomposition of pats). In deeper soil layers below the root zone (5-25 cm), E. coli persistence varied according to soil type, with higher numbers recovered in poorly-drained soils (10(3) to 10(4) cells g-1 dry soil) than in well-drained soils (< 10(2) cells g-1 dry soil). A preliminary analysis of 38 partial uidA sequences of E. coli from pat and soils highlighted a cluster containing sequences only found in this work. Overall, this study raises the possibility that fecal E. coli could have formed a naturalized (sub)population, which is now part of the indigenous soil community of alpine pasture grasslands, the soil thus representing an environmental reservoir of E. coli.
机译：在奶牛高山草原土壤的情况下，我们对现场大肠杆菌（E. coli）生态学的了解非常有限。在这里，我们的目标是在四个不同的高山牧场单元中监测大肠杆菌在牛拍和下层土壤中的野外存活情况，并确定土壤是否可以构成环境水库。使用选择性培养基，通过MPN枚举大肠杆菌。大肠杆菌在牛拍（10（7）至10（8）细胞g-1干拍）中存活得很好，但牛拍在约2个月内消失。在每个牧场单位中，无论采样日期如何，即从采样时间（即在积雪之后，融雪后立即或在牧草季节（拍拍过程中和拍拍后）。在根部区域（5-25厘米）以下的较深土壤层中，大肠杆菌的持久性随土壤类型而异，在排水不良的土壤中（10（3）至10（4）个细胞g-1干燥土壤）中回收的大肠杆菌数量更多）比排水良好的土壤（<10（2）个细胞g-1干燥土壤）高。初步分析了拍拍和土壤中大肠杆菌的38条uidA部分序列，突出显示了一个仅包含这项工作中发现的序列的簇。总体而言，这项研究提出了粪便大肠杆菌可能形成自然化（亚）种群的可能性，该种群现在已成为高山牧场草地土著土壤群落的一部分，因此土壤代表了大肠杆菌的环境储集层。

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