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首页> 外文期刊>Journal of Molecular Biology >THE EFFICIENCY OF TRANSLATION TERMINATION IS DETERMINED BY A SYNERGISTIC INTERPLAY BETWEEN UPSTREAM AND DOWNSTREAM SEQUENCES IN SACCHAROMYCES CEREVISIAE
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THE EFFICIENCY OF TRANSLATION TERMINATION IS DETERMINED BY A SYNERGISTIC INTERPLAY BETWEEN UPSTREAM AND DOWNSTREAM SEQUENCES IN SACCHAROMYCES CEREVISIAE

机译：翻译过程的终止效率由酿酒酵母中上游和下游序列之间的协同相互作用确定

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In a recent study we found that the efficiency of translation termination could be decreased several hundred fold by altering the local sequence context surrounding stop codons in the yeast Saccharomyces cerevisiae. Suppression of termination was shown to be mediated by near-cognate tRNA mispairing with the termination codon. We have now examined in greater detail how the local sequence context affects the efficiency of translation termination in this organism. Our results indicate that the sequence immediately upstream of the termination codon plays a significant role in determining the efficiency of translation termination. An extended termination sequence (containing the stop codon and the following three nucelotides) was also found to be a major determinant of termination efficiency, with effects attributable to the fourth nucleotide being largely independent of the termination codon. For the UGA and UAA stop codons, the influence of the fourth position on termination efficiency (from most efficient to least efficient termination) was found to be G>U,A>C, while for the UAG codon it was U,A>C>G. These sequence-specific effects on the efficiency of translation termination suggest that polypeptide chain release factor (or another molecule that may play a role in translation termination, such as rRNA) recognizes an extended termination sequence in yeast. A previous study found a statistically significant bias toward certain tetranucleotide sequences (containing the stop codon and the first distal nucleotide) in several organisms. We found that tetranucleotide sequences most frequently used in yeast are among the most efficient at mediating translation termination, while rare tetranucleotide sequences mediate much less efficient termination. Taken together, our results indicate that upstream and downstream components of an extended sequence context act synergistically to determine the overall efficiency of translation termination in yeast. [References: 38]
机译：在最近的研究中，我们发现，通过改变啤酒酵母中终止密码子周围的局部序列背景，翻译终止的效率可能降低数百倍。显示终止的抑制是由与终止密码子的近同源tRNA错配对介导的。现在我们已经更详细地研究了本地序列上下文如何影响该生物中翻译终止的效率。我们的结果表明，紧邻终止密码子上游的序列在确定翻译终止效率中起着重要作用。还发现延伸的终止序列（包含终止密码子和随后的三个核苷酸）是终止效率的主要决定因素，其影响可归因于第四个核苷酸在很大程度上与终止密码子无关。对于UGA和UAA终止密码子，发现第四位对终止效率的影响（从最有效终止到最低效率终止）为G> U，A> C，而对于UAG密码子则为U，A> C > G。这些对翻译终止效率的序列特异性影响表明多肽链释放因子（或可能在翻译终止中起作用的另一种分子，如rRNA）识别酵母中的延伸终止序列。先前的研究发现，在几种生物中，某些四核苷酸序列（包含终止密码子和第一个远端核苷酸）在统计学上具有显着偏倚。我们发现酵母中最常使用的四核苷酸序列是介导翻译终止的最有效序列，而稀有的四核苷酸序列介导的终止效率要低得多。两者合计，我们的结果表明，扩展序列上下文的上游和下游组件协同作用，以确定酵母中翻译终止的总体效率。 [参考：38]

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