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In Process Citation

机译：过程中引用

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The fungal transamidase complex that executes glycosylphosphatidylinositol (GPI) lipid anchoring of precursor proteins has overlapping but distinct sequence specificity compared with the animal system. Therefore, a taxon-specific prediction tool for the recognition of the C-terminal signal in fungal sequences is necessary. We have collected a learning set of fungal precursor protein sequences from the literature and fungal proteomes. Although the general four segment scheme of the recognition signal is maintained also in fungal precursors, there are taxon specificities in details. A fungal big-Pi predictor has been developed for the assessment of query sequence concordance with fungi-specific recognition signal requirements. The sensitivity of this predictor is close to 90%. The rate of false positive prediction is in the range of 0.1%. The fungal big-Pi tool successfully predicts the Gas1 mutation series described by C. Nuoffer and co-workers, and recognizes that the human PLAP C terminus is not a target for the fungal transamidase complex. Lists of potentially GPI lipid anchored proteins for five fungal proteomes have been generated and the hits have been functionally classified. The fungal big-Pi prediction WWW server as well as precursor lists are available at
机译：与动物系统相比，执行前体蛋白的糖基磷脂酰肌醇（GPI）脂质锚定的真菌转酰胺酶复合物具有重叠但独特的序列特异性。因此，需要用于识别真菌序列中C端信号的特定分类群预测工具。我们已经从文献和真菌蛋白质组学中收集了一组学习的真菌前体蛋白序列。尽管在真菌前体中也维持识别信号的一般四段方案，但是在细节上还是有分类群特异性的。已开发出一种真菌big-Pi预测因子，用于评估查询序列与真菌特定的识别信号要求的一致性。该预测变量的灵敏度接近90％。假阳性预测的比率在0.1％的范围内。真菌big-Pi工具成功预测了C. Nuoffer及其同事描述的Gas1突变序列，并认识到人PLAP C末端不是真菌转酰胺酶复合物的靶标。已经生成了用于五个真菌蛋白质组的潜在GPI脂质锚定蛋白的列表，并对命中进行了功能分类。真菌big-Pi预测WWW服务器以及前体列表可从以下网站获得：

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