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首页> 外文期刊>Journal of Molecular Biology >POLYMORPHISM OF BACTERIOPHAGE T7
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POLYMORPHISM OF BACTERIOPHAGE T7

机译：噬菌体T7的多态性

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摘要

For viruses made of nucleic acid and protein, the structure of the protein outer shell. has, in the past, been found to be uniquely determined by the viral genome. However, here, non-denaturing agarose gel electrophoresis of bacteriophage T7 reveals two states of the mature T7 capsid; the conditions of growth are found to alter the population by T7 of these two electrophoretically-defined states. Both stales have been previously observed for a genetically altered T7 and they are observed here for wild-type T7. The average electrical surface charge density of a bacteriophage particle (delta) determines its state; the delta of particles in both states is negative. For a given condition of growth, the population of these two states is influenced by the extent to which the major T7 outer shall protein, p10A, is accompanied by its minor readthrough variant, p10B. Comparison of the two electrophoretic states reveals the following. (1) No difference in radius is present in the outer shell (+/-2%). (2) As the pH of electrophoresis is either increased or decreased from neutrality, the state becomes more highly populated for which delta is greater in magnitude (state 1). By changing the pH, some T7 particles are made to change state. (3) Particles in state 1 adsorb less quickly to host cells than do the particles in the alternative state (state 2). This latter observation suggests the hypothesis that state 1 evolved to reduce the probability of re-initiating an infection when conditions are not favorable for growth. This hypothesis is supported by the observation that, as conditions of growth become apparently more unfavorable, progeny increasingly populate state 1. (C) 1997 Academic Press Limited. [References: 28]
机译：对于由核酸和蛋白质制成的病毒，蛋白质外壳的结构。在过去，已经发现病毒基因组是唯一决定的。然而，在这里，噬菌体T7的非变性琼脂糖凝胶电泳揭示了成熟的T7衣壳的两种状态：发现生长条件通过这两个电泳定义的状态的T7改变了种群。先前已经观察到两种变态的基因改变的T7，在这里已经观察到它们的野生型T7。噬菌体颗粒的平均表面电荷密度（δ）决定了其状态。两种状态下的粒子增量均为负。对于给定的生长条件，这两个州的种群受主要的T7外在蛋白p10A与其次要的通读变体p10B伴随的程度影响。两种电泳状态的比较揭示了以下内容。 （1）外壳没有半径差异（+/- 2％）。 （2）随着电泳pH值从中性增加或降低，该状态的填充量将变得更大，其增量值将更大（状态1）。通过改变pH值，可以使某些T7颗粒改变状态。 （3）状态1的粒子与替代状态（状态2）相比，吸附到宿主细胞的速度较慢。后一种观察结果提出了这样的假设：状态1进化为在条件不利于生长的情况下减少重新引发感染的可能性。该假设得到以下观察的支持，即随着生长条件变得越来越不利，后代越来越多地进入状态1。（C）1997 Academic Press Limited。 [参考：28]

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