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首页> 外文期刊>Journal of neurosurgical sciences >The importance of substance P and calcitonin gene related peptide as vasodilator neuropeptide during acute phase of experimental posthemorrhagic vasospasm.
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The importance of substance P and calcitonin gene related peptide as vasodilator neuropeptide during acute phase of experimental posthemorrhagic vasospasm.

机译：在实验性出血后血管痉挛急性期，P物质和降钙素基因相关肽作为血管舒张神经肽的重要性。

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BACKGROUND: Several immunohistochemical studies conducted in the acute phase following SAH have demonstrated a marked depletion of certain peptides like Substance P (SP), Calcitonin Gene Related Peptide (CGRP) from the adventitia of perivascular nerves. The present experimental study was carried out with the aim of determining whether the depletion of these peptides could be a protection mechanism against the factors which sustain the vasospasm. METHODS: To accomplish this goal, we administered specific antiserum to block the potential effect of neuropeptides (SP and CGRP) prior to SAH. Our study tried also to realize whether difference can be demonstrated between endothelium-dependent (SP) and endothelium-independent vasodilatory mechanisms (CGRP) during the acute phase ofvasospasm. Twenty-three rabbits were divided in 5 experimental groups: Group A included normal control animals, Group B included rabbits who received saline injection prior to SAH, Group C included animals who received preimmune serum, groups D and E included animals who received respectively antiserum against CGRP and against SP prior to SAH. The antisera were administered into the cisterna magna by means of percutaneous suboccipital puncture. After 15 minutes 1 ml of autologous non-heparinized blood was injected in the same way. After 20 minutes the animals were sacrificed by cardiac perfusion. The basilar artery was removed by means of transclival approach and it was included in Epon 812. Mean diameters and luminal areas of the arteries were measured with morphometric method on sections of 2-3 microm of thickness. RESULTS: The results showed a reduced mean diameter and luminal areas in the group B comparing to normal controls of the group A. A marked vasospasm is mainly evident in group D and E. In group C the vasospasm is not significantly different from that of group B. No significant difference was demonstrated between group D and group E. CONCLUSIONS: We can conclude that: 1) the marked depletion of neuropeptides in the early phases of vasospasm represents a functional phenomenon in order to reduce the effectiveness of spasmogenic stimula. In fact the inhibition of the activity of these neuropeptides worses the entity of the vasospasm. 2) During the acute phase of vasospasm the endothelium-dependent vasodilatory mechanism is still functioning. No significant difference in the entity of vasospasm has been demonstrated between inhibition of SP (endothelium-dependent) and CGRP (endothelium-independent). Inactivation of such a mechanism occurs during late phases.
机译：背景：SAH后在急性期进行的一些免疫组织化学研究表明，血管周神经外膜中某些肽（如P物质（SP），降钙素基因相关肽（CGRP））的消耗显着减少。进行本实验研究的目的是确定这些肽的消耗是否可以作为针对维持血管痉挛的因素的保护机制。方法：为了实现这一目标，我们在SAH之前给予了特定的抗血清以阻断神经肽（SP和CGRP）的潜在作用。我们的研究还试图了解在血管痉挛的急性期是否可以证明内皮依赖性（SP）和非内皮依赖性血管舒张机制（CGRP）之间存在差异。将23只兔分为5个实验组：A组包括正常对照动物，B组包括在SAH之前注射生理盐水的兔子，C组包括接受免疫前血清的动物，D组和E组包括分别接受抗血清的动物CGRP和SAH之前针对SP。通过经皮枕下穿刺将抗血清注入大水罐。 15分钟后，以相同方式注射1ml自体非肝素化血液。 20分钟后，通过心脏灌注将动物处死。基底动脉通过跨腹腔入路切除，并包含在Epon 812中。用形态计量学方法在2-3微米厚的切片上测量平均动脉直径和管腔面积。结果：结果显示，与正常对照组相比，B组的平均直径和管腔面积减少。明显的血管痉挛主要在D组和E组明显。C组的血管痉挛与B组无明显差异。 B.在D组和E组之间没有显示出显着差异。结论：我们可以得出以下结论：1）在血管痉挛的早期，神经肽的明显消耗代表一种功能性现象，以减少痉挛性刺激的有效性。实际上，对这些神经肽活性的抑制使血管痉挛的实体恶化。 2）在血管痉挛的急性期，内皮依赖性血管舒张机制仍在起作用。在SP（内皮依赖性）和CGRP（内皮依赖性）的抑制之间没有显示出血管痉挛的显着差异。这种机制的失活发生在后期。

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