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首页> 外文期刊>Journal of Nematology, with Annual of Applied Nematology >Host-Specific Pathogenicity and Genome Differences between Inbred Strains of Meloidogyne hapla
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Host-Specific Pathogenicity and Genome Differences between Inbred Strains of Meloidogyne hapla

机译：根结线虫近交系之间宿主特异性的致病性和基因组差异

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摘要

Five isolates of M. hapla originating from the Netherlands and California were inbred by sequential transfer of single egg masses to produce six strains. Cytological examination showed that oocytes of these strains underwent meiosis and had n = 16 chromosomes. Strains were tested for ability to infect and to develop on several hosts by in vitro assays. The two strains from California infected tomato roots at a higher rate than those from the Netherlands, but no difference among strains was seen for ability to develop on tomato with or without the resistance gene Mi-1. All strains developed on the common bean cultivar Kentucky Wonder, but strains differed in ability to develop on the nematode-resistant cultivar NemaSnap. Strain-specific differences were also seen in ability to infect and to develop on Solanum bulbocastanum clone SB-22. Strain VW13, derived from nematodes treated with the mutagen EMS, was defective in ability to infect tomato and potato roots in vitro. Comparison of DNA using AFLP markers showed an average of 4% of the bands were polymorphic across the six strains, but no correlation was observed between the geographical origin or virulence and DNA polymorphism pattern.
机译：通过顺序转移单个卵团来自交自荷兰和加利福尼亚的五个鼠疫支原体分离株以产生六个菌株。细胞学检查表明，这些菌株的卵母细胞经历了减数分裂并具有n ＝ 16个染色体。通过体外测定测试了菌株在几种宿主上的感染和发育能力。来自加利福尼亚的两种菌株感染番茄根的速度要高于来自荷兰的两种，但在带有或不带有抗性基因Mi-1的番茄上生长的能力之间没有发现差异。所有菌株都在普通的豆品种肯塔基州奇迹上发展，但菌株在抗线虫品种NemaSnap上的发育能力不同。菌株特异的差异也显示在鳞茎茄属克隆SB-22的感染和发育能力上。来源于用诱变剂EMS处理过的线虫的菌株VW13在体外感染番茄和马铃薯根部的能力上存在缺陷。使用AFLP标记对DNA进行的比较显示，在这6个菌株中，平均4％的条带是多态性的，但是在地理来源或毒力与DNA多态性模式之间未发现相关性。

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