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In Process Citation

机译：过程中引用

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The submucous plexus of the guinea pig intestine is a quasi-two-dimensional mammalian neural network that is particularly amenable to study using multiple site optical recording of transmembrane voltage (MSORTV) [Biol. Bull. 183 (1992) 344; J. Neurosci. 19 (1999) 3073]. For several years the potentiometric dye of choice for monitoring the electrical activity of its individual neurons has been di-8-ANEPPS [Neuron 9 (1992) 393], a naphthylstyryl-pyridinium dye with a propylsulfonate headgroup that provides relatively large fluorescence changes during action potentials and synaptic potentials. Limitations to the use of this dye, however, have been its phototoxicity and its low water solubility which requires the presence of DMSO and Pluronic F-127 in the staining solution. In searching for less toxic and more soluble dyes exhibiting larger fluorescence signals, we first tried the dienylstyryl-pyridinium dye RH795 [J. Neurosci. 14 (1994) 2545] which is highly soluble in water. This dye yielded relatively large signals, but it was internalized quickly by the submucosal neurons resulting in rapid degradation of the signal-to-noise ratio. We decided to synthesize a series of naphthylstyryl-pyridinium dyes (di-n-ANEPPDHQ) having the same chromophore as di-8-ANEPPS and the quaternary ammonium headgroup (DHQ) of RH795 (resulting in two positive charges versus the neutral propylsulfonate-ring nitrogen combination), and we tested the di-methyl (JPW3039), di-ethyl (JPW2081), di-propyl (JPW3031), di-butyl (JPW5029), and di-octyl (JPW5037) analogues, all of them soluble in ethanol. We found that the di-propyl (di-3-ANEPPDHQ) and the di-butyl (di-4-ANEPPDHQ) forms yielded the best combination of signal-to-noise ratio, moderate phototoxicity and absence of dye internalization.
机译：豚鼠肠粘膜下丛是一种准二维的哺乳动物神经网络，特别适合使用跨膜电压的多部位光学记录（MSORTV）进行研究。公牛。 183（1992）344; J.神经科学。 19（1999）3073]。数年来，用于监视其单个神经元电活动的电位染料的选择一直是di-8-ANEPPS [Neuron 9（1992）393]，一种萘基苯乙烯基-吡啶鎓染料，带有丙基磺酸根基团，在作用过程中提供相对较大的荧光变化势和突触势。然而，使用这种染料的局限性在于其光毒性和低水溶性，这要求在染色溶液中存在DMSO和Pluronic F-127。为了寻找毒性更大，溶解性更强的荧光信号，我们首先尝试了二烯基苯乙烯基吡啶鎓染料RH795。神经科学。 14（1994）2545]。这种染料产生相对较大的信号，但是它被粘膜下神经元迅速内在化，导致信噪比迅速下降。我们决定合成一系列具有与di-8-ANEPPS相同的生色团和RH795的季铵头基（DHQ）的萘基苯乙烯基-吡啶鎓染料（di-n-ANEPPDHQ）（产生两个正电荷，而相对于中性丙基磺酸盐环氮结合），我们测试了二甲基（JPW3039），二乙基（JPW2081），二丙基（JPW3031），二丁基（JPW5029）和二辛基（JPW5037）类似物，它们都可溶于乙醇。我们发现二丙基（di-3-ANEPPDHQ）和二丁基（di-4-ANEPPDHQ）形式产生了信噪比，中等光毒性和没有染料内在化的最佳组合。

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