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A panel of polymorphic EST-derived microsatellite loci for the bay scallop (Argopecten irradians)

机译:海湾扇贝(Argopecten irradians)的一组多态性EST衍生的微卫星基因座

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The constant progress in aquaculture research has provoked an increasing interest in molecular tools for population analysis, marker-assisted early selection, controlled crosses and fertilization analysis (Weising et al, 1998; Roberts et al., 2005). Micro-satellite markers have proved to be one of the best choices. Microsatellites, or simple sequence repeats (SSRs), are small tandemly repeated sequences that are abundantly scattered throughout a genome (Toth et al., 2000). The advantages of microsatellites include co-dominance, high reproducibility and hypervariability (Jarne & Lagoda, 1996). Consequently, the use of microsatellite markers has expanded considerably over the past decade, both in the number of studies and the number of organisms, primarily due to their facility and power for population genetic analyses (Xu et al., 2001; Senanan et al., 2004). The bay scallop, Argopecten irradians, widely distributed along the Atlantic and Gulf coasts of the United States, is an hermaphroditic bivalve mollusc with a single, large adductor muscle (Roberts et al., 2005). In China, the bay scallop became one of the main cultured bivalves after being transplanted from USA in the early 1980s. Recently, 17 EST-SSRs have been developed (Roberts et al., 2005; Zhan et al., 2005). In this study, we describe the mining of microsatellites via bio-informatic analysis of the bay scallop ESTs, and nine new polymorphic EST-SSRs are also reported.
机译:水产养殖研究的不断进步引起了人们对用于种群分析,标记辅助的早期选择,杂交控制和施肥分析的分子工具的兴趣日益增加(Weising等,1998; Roberts等,2005)。事实证明,微卫星标记是最佳选择之一。微卫星或简单序列重复序列(SSR)是小的串联重复序列,在整个基因组中分布很丰富(Toth等,2000)。微卫星的优势包括共支配性,高再现性和高可变性(Jarne&Lagoda,1996)。因此,在过去的十年中,无论是在研究数量还是在生物数量上,微卫星标记的使用都已大大扩展,这主要是由于它们在种群遗传分析中的便利性和功能(Xu等人,2001; Senanan等人。 ,2004)。海湾扇贝(Argopecten irradians)广泛分布于美国的大西洋和墨西哥湾沿岸,是一种雌雄同体的双壳软体动物,具有单个大的内收肌(Roberts等,2005)。在中国,海湾扇贝在1980年代初从美国移植后成为主要的养殖双壳动物之一。最近,已经开发了17种EST-SSR(Roberts等,2005; Zhan等,2005)。在这项研究中,我们通过海湾扇贝EST的生物信息学分析描述了微卫星的开采,并且还报道了9种新的多态EST-SSR。

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