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首页> 外文期刊>Journal of orthopaedic research >Immunohistochemical analysis of type-X-collagen expression in osteoarthritis of the hip joint.
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Immunohistochemical analysis of type-X-collagen expression in osteoarthritis of the hip joint.

机译：髋关节骨关节炎中X型胶原蛋白表达的免疫组织化学分析。

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Conflicting data have been reported on the spatial distribution of type X-collagen expression in osteoarthritis, and no concise data exist on a possible correlation between type X-collagen expression and clinical and radiological alterations. Well defined clinical and radiological data were compared with histopathological and immunohistochemical findings to investigate the expression of type-X collagen in osteoarthritis of the hip joint. Femoral heads were obtained in toto from 11 patients undergoing routine hip arthroplasty for femoral neck fractures (n = 3) or osteoarthritis (n = 8) and from 13 patients (age: 12 days to 69 years) without any evidence of hip-joint pathology. Whole coronal sections from the femoral head were decalcified for routine histology and immunohistochemical analysis with use of type-specific monoclonal antibodies to type-X collagen. Our results demonstrate that type-X collagen is consistently found in osteoarthritic cartilage and is absent from normal adult cartilage (including the region of calcified cartilage). Except for the occurrence of type-X collagen in the middle zone of articular cartilage in advanced stages of osteoarthritis, there is no specific change in the staining pattern or intensity for the collagen during osteoarthritis, particularly when the staining is related to clinical and radiological parameters. Hardly more than 20% of the extracellular matrix stained for type-X collagen; therefore, we suggest that, in most cases, this type of collagen may not play a direct biomechanical role in the weakening of osteoarthritic cartilage but rather may contribute indirectly to a disturbance of the disc biomechanics by altering matrix-molecule interaction. However, expression of type-X collagen may indicate a change in chondrocyte phenotype that consistently coincides with the formation of chondrocyte clusters, one of the first alterations in osteoarthritis visible on histologic examination.
机译：关于骨关节炎中X型胶原蛋白表达的空间分布的报道相互矛盾，关于X型胶原蛋白表达与临床和放射学改变之间可能的相关性，尚无简明数据。将明确定义的临床和放射学数据与组织病理学和免疫组织化学结果进行比较，以研究X型胶原在髋关节骨关节炎中的表达。共有11例因股骨颈骨折（n = 3）或骨关节炎（n = 8）接受常规髋关节置换术的患者和13例（年龄：12天至69岁）的患者均获得了股骨头，没有任何髋关节病理学证据。使用针对X型胶原的类型特异性单克隆抗体，对股骨头的整个冠状切片进行脱钙以进行常规组织学和免疫组织化学分析。我们的结果表明，X型胶原蛋白在骨关节炎软骨中始终存在，而在正常成人软骨（包括钙化软骨区域）中却不存在。除了在骨关节炎晚期阶段在关节软骨中部出现X型胶原外，骨关节炎期间胶原的染色方式或强度没有特别的变化，特别是当染色与临床和放射学参数有关时。 X型胶原染色的细胞外基质几乎不超过20％;因此，我们建议在大多数情况下，这种类型的胶原蛋白可能不会在骨关节炎的减弱中起直接的生物力学作用，而是可能通过改变基质-分子相互作用而间接地引起椎间盘生物力学的紊乱。然而，X型胶原蛋白的表达可能表明软骨细胞表型的变化与软骨细胞簇的形成一致，这是组织学检查中可见的骨关节炎的首批改变之一。

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