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Single cell dielectric spectroscopy

机译:单细胞介电谱

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摘要

Over the last century a number of techniques have been developed which allow the measurement of the dielectric properties of biological particles in fluid suspension. The majority of these techniques are limited by the fact that they only provide an average value for the dielectric properties of a collection of particles. More recently, with the advent of microfabrication techniques and the Lab-on-a-chip, it has been possible to perform dielectric spectroscopic experiments on single biological particles suspended in physiological media. In this paper we review current methods for single cell dielectric spectroscopy. We also discuss alternative single cell dielectric measurement techniques, specifically the ac electrokinetic methods of dielectrophoresis and electrorotation. Single cell electrical impedance spectroscopy is also discussed with relevance to a microfabricated flow cytometer. We compare impedance spectroscopy data obtained from measurements made using a microfabricated flow cytometer with simulation data obtained using an equivalent circuit model for the device.
机译:在上个世纪,已经开发了许多技术,这些技术可以测量流体悬浮液中生物颗粒的介电性能。这些技术中的大多数受到以下事实的限制:它们仅提供粒子集合的介电特性的平均值。最近,随着微细加工技术和芯片实验室的出现,可以对悬浮在生理介质中的单个生物颗粒进行介电光谱实验。在本文中,我们回顾了单细胞介电谱的当前方法。我们还将讨论替代的单细胞介电测量技术,特别是介电电泳和电旋转的交流电动力学方法。还讨论了与微流式细胞仪有关的单细胞电阻抗光谱。我们将通过使用微型流式细胞仪进行的测量获得的阻抗谱数据与使用设备的等效电路模型获得的仿真数据进行比较。

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