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首页> 外文期刊>Journal of liquid chromatography and related technologies >Preparative separation of isoflavones from soybean by reversed-phase high performance liquid chromatography
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Preparative separation of isoflavones from soybean by reversed-phase high performance liquid chromatography

机译：反相高效液相色谱法从大豆中制备异黄酮

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摘要

As a potential anticancer agent. the isoflavones contained in soy- beans were considered for separation by reversed-phase high per- formance liquid chromatography (RP-HPLC). The mobile phases were the ternary system of ~.ater/acetonitrile/acetic acid. In this work, the four different celumns of Il-Bondapak (0.39 x 30 cm.. 10 ~m),andthe other three empty stainless steel columns (0.39 x 30. .1 x25. I"x 25 cm)packed with 15 ~mC..,packings were used. The experimental variables were the gradient conditions and mobile phase composition. The increased number of gradient steps enabled the separation ofisoflavones in the larger columns. The separation condition .of I" x 25 cm column was determined by experiments of the systematic sequence of the smaller columns. For the largest column. 2 mL of sample was separated with the three gradient steps at }8 mUmin of mobile phas.e flow rate. The isoflavones of daidzein, glycitin: genistin, daidzin,and glyci'tein were identified by LC-MS with the purified components.
机译：作为潜在的抗癌药。反相高效液相色谱法（RP-HPLC）认为可以分离大豆中所含的异黄酮。流动相为〜at /乙腈/乙酸的三元体系。在这项工作中，Il-Bondapak的四个不同体积（0.39 x 30 cm .. 10〜m）和其他三个空的不锈钢柱（0.39 x 30. .1 x25。I“ x 25 cm）装满了15〜实验参数为梯度条件和流动相组成，梯度步骤数的增加使较大的色谱柱能够分离异黄酮。通过实验确定了I“ x 25 cm色谱柱的分离条件较小列的系统顺序。对于最大的列。通过三个梯度步骤以} 8 mUmin的移动相流速分离2 mL样品。用纯化的成分通过LC-MS鉴定了大豆苷元，大豆苷元：黄豆苷元，大豆苷和大豆苷元的异黄酮。

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