Purification of peg-protein conjugates by countercurrent distribution in aqueous two-phase systems

Terasut Sokkumnerd; James T.Hsu

首页> 外文期刊>Journal of liquid chromatography and related technologies >Purification of peg-protein conjugates by countercurrent distribution in aqueous two-phase systems

【24h】

Purification of peg-protein conjugates by countercurrent distribution in aqueous two-phase systems

机译：在水性两相系统中通过逆流分布纯化钉蛋白偶联物

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Purficatin of PEG-protein onjugates by countercurrent distribution(CCD)in aqueous two-phase systems is reported. By usig CCD in 19.36% PEG 3400/14.68% potassium phosphate, we can separate PEG-lysozyme conjugates, which contains an average number of 1.14 PEG per lysozyme, into three peaks with 55 number of transfers. Further analysis of the samples reveals that these three peaks mainy represent unmodified lysozyme, (PEG)_1-lysozyme and (PEG)_2-lysozyme.

机译：报道了在水相两相系统中通过逆流分布（CCD）产生的PEG蛋白的嘌呤抑菌作用。通过在19.36％的PEG 3400 / 14.68％磷酸钾中使用igCCD，我们可以将PEG-溶菌酶结合物（每个溶菌酶平均包含1.14 PEG）分离为三个峰，具有55个转移。样品的进一步分析表明这三个峰主要代表未修饰的溶菌酶，（PEG）_1-溶菌酶和（PEG）_2-溶菌酶。

著录项

来源
《Journal of liquid chromatography and related technologies》 |2000年第4期|共7页
作者
Terasut Sokkumnerd; James T.Hsu;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类分析化学;
关键词

相似文献

外文文献
中文文献
专利

1. Purification of peg-protein conjugates by countercurrent distribution in aqueous two-phase systems [J] . Terasut Sokkumnerd, James T.Hsu Journal of liquid chromatography and related technologies . 2000,第4期

机译：在水性两相系统中通过逆流分布纯化钉蛋白偶联物
2. Purification of proteins with aqueous two-phase solvent systems by countercurrent chromatography [J] . Shibusawa Y., Ito Y. Preparative biochemistry & biotechnology: An international journal for rapid communication . 1998,第2期

机译：逆流色谱法在含水两相溶剂系统中纯化蛋白质
3. Countercurrent Chromatographic Purification of Polysaccharides from Achyranthes bidentata with an Aqueous Two-Phase System Using a Cross-Axis Coil Planet Centrifuge [J] . Zhimao Chao, Yoichi Shibusawa, Heisaburo Shindo, Journal of liquid chromatography and related technologies . 2003,第12期

机译：两相水系统使用横轴线圈行星式离心机逆流色谱纯化牛膝多糖
4. PROTEIN PARTITIONING IN POLYETHYLENGLYCOL/POTASSIUM PHOSPHORIC ACID AQUEOUS TWO-PHASE SYSTEMS FOR PURIFICATION OF MEMBRANE PROTEIN [C] . C.Gao, rnF.Shi, rnL.Cai, Frontiers on Separation Science and Technology . 2004

机译：蛋白质在聚乙烯乙二醇/磷酸钾水溶液两相系统中的分离以纯化膜蛋白
5. Purification of human antibody from transgenic corn using aqueous two-phase systems [D] . Lee, Jinwook 2006

机译：使用水性两相系统从转基因玉米中纯化人抗体
6. Studies of protein-protein interaction using countercurrent distribution in aqueous two-phase systems. Partition behaviour of six Calvin-cycle enzymes from a crude spinach (Spinacia oleracea) chloroplast extract. [O] . L O Persson, G Johansson 1989

机译：研究在水两相系统中使用逆流分布的蛋白质-蛋白质相互作用。粗菠菜叶绿体提取物中六种加尔文循环酶的分配行为。
7. Isolation of Plasma Membranes from the Nervous System by Countercurrent Distribution in Aqueous Polymer Two-Phase Systems [O] . Jens Schindler, Hans Gerd Nothwang 2015

机译：水相聚合物两相体系中逆流分布从神经系统中分离血浆膜

Purification of peg-protein conjugates by countercurrent distribution in aqueous two-phase systems

摘要

著录项

相似文献

相关主题

期刊订阅