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首页> 外文期刊>Journal of Parasitology >Isolation of Toxoplasma gondii From the Keel-Billed Toucan (Ramphastos sulfuratus) From Costa Rica
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Isolation of Toxoplasma gondii From the Keel-Billed Toucan (Ramphastos sulfuratus) From Costa Rica

机译:从哥斯达黎加龙骨嘴巨嘴鸟(Ramphastos sulfatus)中分离弓形虫

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摘要

Pectoral muscles from a captive keel-billed toucan (Ramphastos sulfuratus) from Cost Rica were fed to a Toxoplasma gondii-free cat, and the cat shed oocysts. Laboratory mice fed these oocysts developed antibodies to T. gondii in their sera and T. gondii tissue cysts in their brains. The DNA extracted from the brains of infected mice was characterized using 10 polymerase chain reaction-restricted fragment length polymorphic markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico). The isolate designated TgRsCr1 was found to be non-clonal with Type I, II, and III alleles at different loci. This is the first isolation of T. gondii from this host.
机译:将来自Cost Rica的圈养龙嘴大嘴鸟巨嘴鸟(Ramphastos sulphatus)的胸肌喂给无弓形虫的猫,该猫脱落了卵囊。喂食了这些卵囊的实验小鼠的血清中产生了针对弓形虫的抗体,大脑中产生了弓形虫组织囊肿。使用10种聚合酶链反应限制的片段长度多态性标记(SAG1,SAG2,SAG3,BTUB,GRA6,c22-8,c29-2,L358,PK1和Apico)对从感染小鼠大脑中提取的DNA进行表征。发现命名为TgRsCr1的分离株是非克隆的,在不同位点具有I,II和III型等位基因。这是从该宿主中首次分离到弓形虫。

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