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首页> 外文期刊>Journal of submicroscopic cytology and pathology >An ex vivo model to study the monocyte-endothelial cell interaction in the prelesional stage of experimentally-induced atherogenesis in hamster.
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An ex vivo model to study the monocyte-endothelial cell interaction in the prelesional stage of experimentally-induced atherogenesis in hamster.

机译：一种体外模型，用于研究仓鼠实验诱导的动脉粥样硬化病变前期的单核细胞-内皮细胞相互作用。

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We imagined an ex vivo atherogenic model consisting in hypercholesterolemic versus normocholesterolemic aortic arch rings or sigmoid valves incubated in cell culture conditions with human monocytes. Normal tissues did not show any attached monocytes. On the atherogenic aortic arch three different aspects were observed: a) there were no monocytes attached on a normal zone; b) many monocytes adhered to the endothelium on a thickened area, with intimal smooth muscle cells infiltration, and c) the greatest number of attached monocytes was seen in mechanically injured zones of the aortic arch, where the subendothelial area was totally exposed. Immunohisto- and immunocytochemistry for LFA-1 revealed the presence of this leukocyte integrin on monocyte plasma membrane. The labelled monocyte had an activated shape, with pseudopodes extended over the endothelial cells and the anti-LFA-1 antibody coupled with colloidal gold decorating areas apposing to a morphologically modified endothelium. In conclusion, the exvivo model reproduced the in vivo situations where the monocytes adhere to the modified endothelium covering the thickened areas of hypercholesterolemic aortic wall; they express at least one of the adhesion integrins, namely LFA-1. This study intended to contribute, at least in part, to the understanding of some mechanisms governing the monocyte-endothelial cell interactions in hypercholesterolemia.
机译：我们想象了一个体外致动脉粥样硬化模型，该模型由高胆固醇血症对正常胆固醇的主动脉弓环或乙状结肠瓣膜在与人单核细胞的细胞培养条件下孵育而成。正常组织未显示任何附着的单核细胞。在动脉粥样硬化的主动脉弓上，观察到三个不同方面：a）在正常区域没有附着单核细胞; b）许多单核细胞在增厚的区域附着在内皮上，内膜平滑肌细胞浸润，并且c）在主动脉弓的机械损伤区域（内皮下区域完全暴露），附着的单核细胞数量最多。 LFA-1的免疫组织化学和免疫细胞化学分析显示该白细胞整合素在单核细胞质膜上的存在。标记的单核细胞具有激活的形状，假足在内皮细胞上延伸，抗LFA-1抗体与胶体金修饰区域相结合，该区域与形态上修饰的内皮细胞相对应。总之，离体模型重现了体内情况，其中单核细胞粘附在修饰的内皮上，覆盖了高胆固醇血症主动脉壁的增厚区域。它们表达至少一种粘附整联蛋白，即LFA-1。这项研究旨在至少部分有助于对高胆固醇血症中控制单核细胞-内皮细胞相互作用的某些机制的理解。

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