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首页> 外文期刊>Journal of Stored Products Research >Enhanced proteolytic and cellulolytic activity in insecticide-resistant strains of the maize weevil, Sitophilus zeamais.
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Enhanced proteolytic and cellulolytic activity in insecticide-resistant strains of the maize weevil, Sitophilus zeamais.

机译：在玉米象鼻虫Sitophilus zeamais的抗杀虫剂菌株中增强的蛋白水解和纤维素分解活性。

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Insecticide resistance is frequently associated with fitness costs in the absence of insecticides, but extended and intense past selection with these compounds may favor the evolution of fitness modifier genes that mitigate such costs. Insecticide resistance without fitness cost was associated with greater accumulation of total proteins and carbohydrates in a strain of the maize weevil (Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae)). Increased energy reserves may be due to an accumulation of carbohydrates and proteins because of increased digestive efficiency. To test this hypothesis, proteases and enzymes (cellulase and the pectinases polygalacturonase and pectin lyase), which enable insects to access the nutrients, were used to compare digestive efficiency in insecticide-susceptible and -resistant strains of the maize weevil. A canonical variate analysis indicated significant differences among the strains in enzyme activities, and kinetic parameters were calculated. Serine- and cysteine-proteinases as well as cellulase activities were smaller in susceptible than resistant strains. In addition, the esterolytic activity of serine-proteinases was most increased in the insecticide-resistant strain exhibiting a fitness disadvantage associated with insecticide resistance. Overall, enzymes in the insecticide-resistant strains had increased serine- and cysteine-proteolytic and cellulolytic activity, and kinetic parameters suggested that cysteine-proteinase and cellulase activities were more important in mitigating the cost of insecticide resistance in maize weevil strains.
机译：在没有杀虫剂的情况下，抗药性通常与适应性成本有关，但是对这些化合物的长期选择和长期选择可能有利于减轻适应性修饰基因的进化。在没有适应性费用的情况下，抗药性与玉米象鼻虫（Sitophilus zeamais Motschulsky（鞘翅目：Curculionidae））中总蛋白质和碳水化合物的积累有关。能量储备增加可能是由于消化效率提高导致碳水化合物和蛋白质的积累。为了检验这一假设，使用蛋白酶和酶（纤维素酶和果胶酶聚半乳糖醛酸酶和果胶裂解酶）来使昆虫获得营养，并比较了玉米象鼻虫对杀虫剂敏感和耐药的菌株的消化效率。典范变量分析表明菌株之间酶活性的显着差异，并计算了动力学参数。易感菌株中的丝氨酸和半胱氨酸蛋白酶以及纤维素酶活性要比抗性菌株小。另外，丝氨酸蛋白酶的酯水解活性在抗虫剂菌株中增加最多，表现出与抗虫剂相关的适应性缺点。总体而言，抗药性菌株中的酶具有增加的丝氨酸和半胱氨酸蛋白水解和纤维素分解活性，动力学参数表明半胱氨酸蛋白酶和纤维素酶活性对于降低玉米象鼻虫菌株的抗药性成本更为重要。

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