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首页> 外文期刊>Journal of the Society for Gynecologic Investigation >Nitric oxide synthase isoforms in the rat kidney during pregnancy.
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Nitric oxide synthase isoforms in the rat kidney during pregnancy.

机译：怀孕期间大鼠肾脏中的一氧化氮合酶同工型。

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OBJECTIVE: Nitric oxide mediates renal vasodilation and hyperfiltration during pregnancy in conscious rats through the endothelin B (ETB) receptor subtype. We tested the hypothesis that immunoreactive levels of endothelial nitric oxide synthase (eNOS) would be greater in the kidneys of midterm pregnant rats compared with virgin rats. METHODS: We studied midterm pregnancy because renal plasma flow and glomerular filtration rate are maximal at this gestational stage. Western analysis was used to determine the level of eNOS in the three major zones of the kidney-inner medulla, outer medulla, and cortex-and in isolated small renal arteries, and in purified renal microvessels from the cortex. RESULTS: There were no significant differences in eNOS expression between virgin and midterm pregnant rats in any of those renal tissues, regardless of whether immunoreactivity was expressed as arbitrary densitometry units, as "microg placental equivalents" interpolated from the linear portion of a dose-response curve of placental villous protein (2.5-30 microg, positive control) run concurrently on each gel, or normalized for beta-actin. We also investigated other NOS isoforms. In particular, immunoreactive neuronal NOS (nNOS) was detectable in the inner and outer medulla, but it was not significantly different between groups. nNOS immunoreactivity was below the level of detection in the cortex, but mRNA expression was not significantly different between pregnant and virgin rats by reverse transcriptase polymerase chain reaction. CONCLUSION: Our results suggest that an increase in eNOS isoform mass does not contribute to the endothelin and NO-dependent renal vasodilation in rat gestation.
机译：目的：一氧化氮通过ETB受体亚型介导清醒大鼠的肾脏血管舒张和超滤作用。我们测试了以下假设：相比于原始大鼠，中期妊娠大鼠肾脏中内皮一氧化氮合酶（eNOS）的免疫反应水平更高。方法：我们研究了中期妊娠，因为在此妊娠阶段，肾血浆流量和肾小球滤过率最高。 Western分析用于确定肾内髓质，髓质外层和皮层三个主要区域以及孤立的小肾动脉和皮层中纯化的肾微血管中eNOS的水平。结果：无论免疫反应性是否表示为任意的光密度测定单位，即从剂量反应线性部分中插入的“微胎盘等效物”，原始和中期妊娠大鼠在任何肾脏组织中eNOS的表达均无显着差异。胎盘绒毛蛋白（2.5-30微克，阳性对照）的曲线在每种凝胶上同时运行，或针对β-肌动蛋白标准化。我们还研究了其他NOS亚型。特别是，在内侧和外侧延髓中都可以检测到免疫反应性神经元NOS（nNOS），但两组之间的差异无显着性。 nNOS免疫反应性低于皮层中的检测水平，但是通过逆转录酶聚合酶链反应，在怀孕和处女大鼠之间，mRNA表达没有显着差异。结论：我们的结果表明，eNOS同工型质量的增加对大鼠妊娠过程中的内皮素和NO依赖性肾血管舒张没有贡献。

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