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首页> 外文期刊>Journal of the Society for Gynecologic Investigation >Ontogeny of vascular growth factors in perinatal sheep myocardium.
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Ontogeny of vascular growth factors in perinatal sheep myocardium.

机译：围产期绵羊心肌中血管生长因子的个体发育。

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OBJECTIVE: To examine developmental changes in myocardial gene expression of previously identified regulators of vascular growth. METHODS: Ovine left (LV) and right ventricle (RV) samples were obtained at four time points: 95 days' and 140 days' gestation (term = 145 days) and 7 days and 8 weeks postnatally. mRNA and protein levels of vascular endothelial growth factor (VEGF), its respective receptors (Flk-1 and Flt-1), basic fibroblast growth factor (bFGF), transforming growth factor-beta(1) (TGF-beta1), and endothelial nitric oxide synthase (eNOS) were measured at these different time points. RESULTS: RV but not LV VEGF mRNA levels decreased postnatally, although VEGF protein expression remained unchanged after birth. Flt-1 mRNA expression was divergent between ventricles, although the protein expression pattern was similar in RV and LV, decreasing with maturation. RV and LV Flk-1 mRNA decreased between 95 days and 140 days, remaining stable thereafter, while protein levels only decreased after birth. bFGF protein levels were highest in the LV at 140 days, and decreased after birth but remained unchanged in the RV throught the period examined. TGF-beta1 and eNOS levels were highest early in gestation, decreasing with maturation in both ventricles. CONCLUSION: Developmentally regulated ventricle-specific expression of VEGF, Flt-1, Flk-1, TGF-beta1, bFGF, and eNOS was demonstrated in the ovine myocardium. These findings suggest these proteins may participate in coronary vascular remodeling during the perinatal period and underscore the importance of studying the relationships among transcription factors, target genes, and anatomic/physiologic changes in the whole animal.
机译：目的：检查先前确定的血管生长调节剂的心肌基因表达的发育变化。方法：在四个时间点分别采集了羊的左（LV）和右心室（RV）样本：妊娠95天和140天（足月145天）以及产后7天和8周。血管内皮生长因子（VEGF），其各自的受体（Flk-1和Flt-1），碱性成纤维细胞生长因子（bFGF），转化生长因子β（1）（TGF-beta1）和内皮的mRNA和蛋白水平在这些不同的时间点测量一氧化氮合酶（eNOS）。结果：尽管出生后VEGF蛋白表达保持不变，但出生后RV而不是LV VEGF mRNA水平下降。 Flt-1 mRNA的表达在脑室之间有差异，尽管RV和LV中的蛋白表达模式相似，但随着成熟而降低。 RV和LV Flk-1 mRNA在95天至140天之间下降，此后保持稳定，而蛋白质水平仅在出生后下降。 bFGF蛋白水平在LV在140天时最高，在出生后下降，但在整个检查期间在RV中保持不变。 TGF-beta1和eNOS水平在妊娠早期最高，在两个心室中均随着成熟而降低。结论：在绵羊心肌中，VEGF，Flt-1，Flk-1，TGF-β1，bFGF和eNOS受到发育调节的心室特异性表达。这些发现表明，这些蛋白质可能在围产期参与了冠状动脉的重塑，并强调了研究整个动物中转录因子，靶基因和解剖/生理变化之间关系的重要性。

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