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首页> 外文期刊>Journal of Turfgrass Management >Transgenic creeping bentgrass (Agrostis palustris Huds.) plants regenerated from protoplasts.
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Transgenic creeping bentgrass (Agrostis palustris Huds.) plants regenerated from protoplasts.

机译：从原生质体再生的转基因genic草（草bent）。

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摘要

Electroporation was used to introduce the hygromycin phosphotransferase reporter gene into creeping bentgrass (Agrostis palustris [A. stolonifera var. palustris]) protoplasts isolated from embryogenic suspension cells. After the subsequent selections with 50 mg hygromycin/litre, several plantlets were regenerated from hygromycin resistant calluses. PCR and Southern hybridization analysis of randomly chosen plants indicated that the transgene was integrated into their genomes and no false positiveswere found. The results suggested that hygromycin phosphotransferase gene was effective selectable marker gene for the selection of transformed cells. Overall frequency for hygromycin resistant colony formation in this report was 1-3 X 10 -6 based on thenumber of treated protoplasts.
机译：电穿孔用于将潮霉素磷酸转移酶报告基因导入从胚发生性悬浮细胞中分离出来的蠕生草（原生草（Agrostis palustris [A. stolonifera var。palustris]）原生质体中。在随后用50mg潮霉素/升进行选择后，从潮霉素抗性的愈伤组织中再生出几株小植株。对随机选择的植物进行的PCR和Southern杂交分析表明，转基因已整合到其基因组中，未发现假阳性。结果提示潮霉素磷酸转移酶基因是选择转化细胞的有效选择标记基因。基于治疗的原生质体数量，本报告中潮霉素抗性菌落形成的总频率为1-3 X 10 -6。

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