• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Turfgrass Management >Electrophoretic evaluation of esterase isozymes from turfgrass seed blends and mixtures.
【24h】

Electrophoretic evaluation of esterase isozymes from turfgrass seed blends and mixtures.

机译：草坪草种子混合物和混合物中酯酶同工酶的电泳评估。

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Turfgrass seeds are commonly blended to improve performance. However, once physically blended, no fast, reliable technique is available to identify the cultivars and the proportion of each cultivar in the blend. The objective of this study was to isolate specific species and cultivars in seed blends or mixtures through examination of esterase banding patterns on electrophoretic gels, and to compare band intensities unique to each cultivar or species when blended in increasing or decreasing proportions. Two cultivars of Kentucky bluegrass (Poa pratensis) were blended by weight in proportions ranging from 0 to 100%. Protein was extracted, separated on isoelectric focusing gels (pH gradient 3-9) and stained for esterase activity. Mixtures of fine fescue (Festuca rubra subsp. commutata [F. nigrescens] and F. ovina subsp. duriuscula [Festuca trachyphylla]) species only and mixtures of both fine fescue species and Kentucky bluegrass cultivars were prepared in the same manner. Gels were visually analysed for the presence of characteristic bands unique to each cultivar or species and for intensities of characteristic bands when cultivar proportions were varied within a seed blend or mixture. The results indicated that visual discriminations could be made between cultivars and species in turfgrass blends or mixtures and that bands unique to a cultivar or species varied in intensity with their concentration in a blend or mixture.
机译：通常将草皮种子混合以提高性能。但是，一旦进行物理混合，就没有快速，可靠的技术来鉴定混合物中每种品种的品种和比例。这项研究的目的是通过检查电泳凝胶上的酯酶谱带模式来分离种子混合物或混合物中的特定物种和品种，并比较每种品种或物种按递增或递减比例混合时独特的条带强度。将两个品种的肯塔基州早熟禾（Poa pratensis）按重量混合，比例为0％至100％。提取蛋白质，在等电聚焦凝胶上分离（pH梯度3-9），并对其酯酶活性进行染色。仅以优良的羊茅种（Festuca rubra commutata [F. nigrescens]和F. ovina duriuscula [Festuca trachyphylla]）的混合物进行混合，并以相同的方式制备了优良的羊茅种和肯塔基州早熟禾品种。对凝胶进行视觉分析，以了解每种品种或物种所特有的特征带，以及在种子混合物或混合物中改变品种比例时特征带的强度。结果表明，可以在草皮草混合物或混合物中对品种和品种进行视觉辨别，并且品种或品种特有的条带强度随混合物或混合物中的浓度而变化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号