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首页> 外文期刊>Colture Protette: CP >Bioagronomic evaluation of a micropropagated clone of Campanula medium.
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Bioagronomic evaluation of a micropropagated clone of Campanula medium.

机译：风铃培养基微繁殖克隆的生物农学评估。

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摘要

Explants of Campanula medium were grown on MS-based medium and used in a mass multiplication program. A field trial with clonal material acclimatized to in vivo conditions was carried out from September to June. The plants were subjected to different temperature treatments before transplanting (0 or 6 weeks at 5 degrees C) or to different photoperiods after transplanting (ND or LD conditions). For LD conditions, a day lengthening treatment was supplied by incandescent lamps with a light intensity of 60-100 lux. The plants flowered in May, irrespective of the temperature or photoperiod treatment. The best results were achieved for plants without low temperature treatment maintained in ND conditions. Eight flowers/plant, out of a total yield of 12.2 flowers/plant (nearly 70 stems/m2 of cultivated soil), showed a higher quality corresponding to a stem height >60 cm and more than 15 bells on the inflorescence. High homogeneity of the morphological characters of the flowers, resembling the superior characters of the original plant, was observed. Micropropagation was successfully carried out and rooting of the microcuttings was performed in perlite with gradual adaptation to "in vivo" conditions..
机译：风铃培养基的外植体在基于MS的培养基上生长，并用于大规模繁殖程序。 9月至6月进行了针对克隆材料适应体内条件的现场试验。在移植前（5摄氏度下0或6周）对植物进行不同的温度处理，或在移植后（ND或LD条件）对植物进行不同的光周期处理。对于LD条件，通过光强度为60-100 lux的白炽灯进行日间延长治疗。不论温度或光周期处理如何，植物均于5月开花。没有在ND条件下保持低温处理的植物获得了最佳结果。每株植物总产量为12.2朵花（栽培土壤近70茎/ m2）中有八朵花，表现出更高的质量，对应于茎高> 60 cm且花序大于15铃。观察到花的形态特征具有很高的同质性，类似于原始植物的优良特征。成功地进行了微繁殖，并在逐渐适应“体内”条件的珍珠岩中生根了微切屑。

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