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首页> 外文期刊>Colloids and Surfaces, B. Biointerfaces >Protein resistant polyurethane surfaces by chemical grafting of PEO: amino-terminated PEO as grafting reagent
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Protein resistant polyurethane surfaces by chemical grafting of PEO: amino-terminated PEO as grafting reagent

机译：通过化学接枝PEO制成的耐蛋白质聚氨酯表面：氨基端接的PEO作为接枝剂

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摘要

The objective of this work was to gain a better understanding of the mechanism of resistance to protein adsorption of surfaces grafted with poly(ethylene oxide) (PEO). A polyurethane-urea was used as a substrate to which PEO was grafted. Grafting was carried out by introducing isocyanate groups into the surface followed by reaction with amino-terminated PEO. Surfaces grafted with PEO of various chain lengths (PUU-NPEO) were prepared and characterized by water contact angle and X-ray photoelectron spectroscopy (XPS). XPS data indicated higher graft densities on the PUU-NPEO surfaces than on analogous surfaces prepared using hydroxy-PEO (PUU-OPEO) as reported previously [J.G. Archambault, J.L. Brash, Colloids Surf. B: Biointerf. 33 (2004) 111-120]. Protein adsorption experiments using radiolabeled myoglobin, concanavalin A, albumin, fibrinogen and ferritin as single proteins in buffer showed that adsorption was reduced on the PEO-grafted surfaces by up to 95% compared to the control. Adsorption decreased with increasing PEO chain length and reached a minimum at a PEO MW of 2000. Adsorption levels on surfaces with 5000 and 2000 MW grafts were similar. There was no clear effect of protein size on resistance to protein adsorption. Adsorption on the PUU-NPEO surfaces was significantly lower than on the corresponding PUU-OPEO surfaces, again suggesting higher graft densities on the former. Adsorption of fibrinogen from plasma was also greatly reduced on the grafted surfaces. From analysis (SDS-PAGE, immunoblotting) of the proteins eluted after plasma exposure, it was found that the grafted surfaces and the unmodified substrate adsorbed the same proteins in roughly the same proportions, suggesting that adsorption to the PEO surfaces occurs on patches of bare substrate. The PEO grafts did not apparently cause differential access to the substrate based on protein size. (C) 2004 Elsevier B.V. All rights reserved.
机译：这项工作的目的是为了更好地理解接枝聚环氧乙烷（PEO）的表面对蛋白质吸附的抗性机理。聚氨酯脲用作接枝PEO的基材。通过将异氰酸酯基引入表面，然后与氨基封端的PEO反应进行接枝。制备了接有各种链长的PEO（PUU-NPEO）的表面，并通过水接触角和X射线光电子能谱（XPS）对其进行了表征。 XPS数据表明，PUU-NPEO表面的接枝密度高于以前使用羟基-PEO（PUU-OPEO）制备的类似表面[J.G. Archambault，J.L。Brash，胶体冲浪。 B：Biointerf。 33（2004）111-120]。使用放射性标记的肌红蛋白，伴刀豆球蛋白A，白蛋白，纤维蛋白原和铁蛋白作为缓冲液中的单个蛋白质进行的蛋白质吸附实验表明，与对照相比，PEO移植表面的吸附减少了多达95％。吸附随着PEO链长度的增加而降低，并在2000的PEO MW达到最低。在5000和2000 MW接枝的表面上的吸附水平相似。蛋白质大小对蛋白质吸附的抵抗力没有明显的影响。在PUU-NPEO表面上的吸附明显低于在相应的PUU-OPEO表面上，再次表明在前者上的接枝密度更高。在移植表面上，血浆中血纤蛋白原的吸附也大大减少。通过血浆暴露后洗脱的蛋白质的分析（SDS-PAGE，免疫印迹）发现，嫁接的表面和未修饰的底物以大致相同的比例吸附相同的蛋白质，这表明对PEO表面的吸附发生在裸露的斑块上基质。基于蛋白质大小，PEO移植物显然并未引起对底物的差异进入。 （C）2004 Elsevier B.V.保留所有权利。

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