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首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Biodesulfurization of dibenzothiophene by a newly isolated Rhodococcus erythropolis strain
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Biodesulfurization of dibenzothiophene by a newly isolated Rhodococcus erythropolis strain

机译：新分离的红球红球菌菌株对二苯并噻吩的生物脱硫

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A new dibenzothiophene (DBT) desulfurizing bacterium was isolated from oil-contaminated soils in Iran. HPLC analysis and PCR-based detection of the presence of the DBT desulfurization genes (dszA, dszB and dszC) indicate that this strain converts DBT to 2-hydroxybiphenyl (2-HBP) via the 4S pathway. The strain, identified as Rhodococcus erythropolis SHT87, can utilize DBT, dibenzothiophene sulfone, thiophene, 2-methylthiophene and dimethylsulfoxide as a sole sulfur source for growth at 30 degrees C. The maximum specific desulfurization activity of strain SHT87 resting cells in aqueous and biphasic organic-aqueous systems at 30 degrees C was determined to be 0.36 and 0.47 mu mol 2-HBP min(-1) (g dry cell)(-1), respectively. Three mM DBT was completely metabolized by SHT87 resting cells in the aqueous and biphasic systems within 10 h. The rate and the extent of the desulfurization reaction by strain SHT87 suggest that this strain can be used for the biodesulfurization of diesel oils.
机译：从伊朗的石油污染土壤中分离出一种新的二苯并噻吩（DBT）脱硫细菌。 HPLC分析和基于DBT脱硫基因（dszA，dszB和dszC）的基于PCR的检测表明，该菌株通过4S途径将DBT转化为2-羟基联苯（2-HBP）。鉴定为红球菌SHT87的菌株可以利用DBT，二苯并噻吩砜，噻吩，2-甲基噻吩和二甲基亚砜作为唯一的硫源在30摄氏度下生长。在水相和两相有机相中，SHT87静止细胞的最大比值脱硫活性-在30摄氏度下的水系统分别确定为0.36和0.47μmol 2-HBP min（-1）（g干电池）（-1）。在水和双相系统中，SHT87静止细胞在10小时内将3 mM DBT完全代谢。 SHT87菌株的脱硫反应速率和程度表明该菌株可用于柴油的生物脱硫。

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