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首页> 外文期刊>Biomacromolecules >Composite Nanoparticles Based on Hyaluronic Acid Chemically Cross-Linked with alpha,beta-Polyaspartylhydrazide
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Composite Nanoparticles Based on Hyaluronic Acid Chemically Cross-Linked with alpha,beta-Polyaspartylhydrazide

机译：透明质酸与α，β-聚天冬氨酰肼化学交联的复合纳米粒子

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摘要

In this paper, new composite nanoparticles based on hyaluronic acid (HA) chemically cross-linked with alpha,beta-polyaspartylhydrazide (PAHy) were prepared by the use of a reversed-phase microemulsion technique. HA- PAHy nanoparticles were characterized by FT-IR spectroscopy, confirming the occurrence of the chemical cross-linking, dimensional analysis, and transmission electron micrography, showing a sub-micrometer size and spherical shape. Zeta potential measurements demonstrated the presence of HA on the nanoparticle surface. A remarkable affinity of the obtained nanoparticles toward aqueous media that simulate some biological fluids was found. Stability studies showed the absence of chemical degradation in various media, while in the presence of hyaluronidase, a partial degradation occurred. Cell compatibility was evaluated by performing in vitro assays on human chronic myelogenous leukaemia cells (K-562) chosen as a model cell line and a haemolytic test. HA-PAHy nanoparticles were also able to entrap 5-fluorouracil, chosen as a model drug, and release it in a simulated physiological fluid and in human plasma with a mechanism essentially controlled by a Fickian diffusion.
机译：本文采用反相微乳液技术制备了以透明质酸（HA）与α，β-聚天冬氨酰肼（PAHy）化学交联的新型复合纳米粒子。 HA-PAHy纳米颗粒通过FT-IR光谱进行了表征，证实了化学交联，尺寸分析和透射电子显微照片的出现，显示出亚微米尺寸和球形。 Zeta电势测量证明纳米颗粒表面上存在HA。发现获得的纳米粒子对模拟某些生物流体的水性介质具有显着的亲和力。稳定性研究表明，在各种培养基中均未发生化学降解，而在存在透明质酸酶的情况下，发生了部分降解。通过对选择作为模型细胞系的人类慢性骨髓性白血病细胞（K-562）进行体外测定和溶血试验，评估细胞的相容性。 HA-PAHy纳米粒子还能够捕获被选作模型药物的5-氟尿嘧啶，并通过基本受菲克扩散控制的机制将其释放到模拟的生理液和人体血浆中。

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